Abstract
Exposure ofToxoplasma trophozoites to ultraviolet light (UV; 2,539 Å) remarkably inhibited intracellular multiplication of the toxoplasmas within cultured mouse peritoneal macrophages. These toxoplasmas possessed the ability to induce normal parasitophorous vacuoles (PV) and underwent gradual degeneration in the PV without participation of host-cell lysosomes. Apparently, the basic conformation of the PV, i.e., the association of mitochondria, rough endoplasmic reticulum, and microtubules of the host cell and the presence of microvillous infoldings, was maintained as seen under the electron microscope even after the toxoplasmas had died within the PV. Even PV, in which debris of the toxoplasmas could be observed, did not show the sign of fusion with ferritin-labeled secondary lysosomes.
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References
Anderson SE Jr, Remington JS (1974) Effect of normal and activated human macrophages onToxoplasma gondii. J Exp Med 139:1154–1174
Armstrong JA, Hart PD (1971) Response of cultured macrophages toMycobacterium tuberculosis with observation on fusion of lysosomes with phagosomes. J Exp Med 134:713–740
Borges JS, Johnson WD Jr (1975) Inhibition of multiplication ofToxoplasma gondii by human monocytes exposed to T-lymphocyte products. J Exp Med 141:483–496
Endo T, Nishikawa H, Kobayashi A, Hayashi S (1978) Restoration of the fusibility of the parasitophorous vacuole containingToxoplasma with lysosome by the use of chlorpromazine and dibucaine. Proc Japanese-German Coop Symp Protozool Dis 1:125–131
Grimwood BG (1980) InfectiveToxoplasma gondii trophozoites attenuated by ultraviolet irradiation. Infect Immun 28:532–535
Hart PD, Armstrong JA (1974) Strain virulence and the lysosomal response in macrophages infected withMyobacterium tuberculosis. Infect Immun 10:742–746
Howard-Flanders P (1968) DNA repair. Annu Rev Biochem 37:175–200
Jones TC, Hirsch JG (1972) The interaction betweenToxoplasma gondii and mammalian cells. 2. The absence of lysosomal fusion with phagocytic vacuoles containing living parasites. J Exp Med 136:1173–1194
Sethi KK, Pelster B, Suzuki N, Piekarski G, Brandis H (1975) Immunity toToxoplasma gondii induced in vitro in non-immune mouse macrophages with specifically immune lymphocytes. J Immunol 115:1151–1158
Trump BF (1975) The network of intracellular membranes. In: Weissmann G (ed) Cell membranes. HP Publishing, New York, p 123–133
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Endo, T., Pelster, B. & Piekarski, G. Infection of murine peritoneal macrophages withToxoplasma gondii exposed to ultraviolet light. Z. Parasitenkd. 65, 121–129 (1981). https://doi.org/10.1007/BF00929177
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DOI: https://doi.org/10.1007/BF00929177