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Ascorbic acid prevents lipid peroxidation and oxidative damage of proteins in guinea pig extrahepatic tissue microsomes

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Abstract

It has recently been indicated that in the absence of free iron, NADPH initiates oxidative damage of proteins in guinea pig liver microsomes and also lipid peroxidation and protein damage in cardiac microsomes and that ascorbic acid specifically inhibits both the lipid peroxidation and protein damage [Mukhopadhyay CK, Chatterjee IB: J Biol Chem 269: 13390–13397, 1994; Mukhopadhyay Met al.: Mol Cell Biochem 126: 69–75, 1993]. In this paper we demonstrate that Fe(III)-independent NADPH-initiated lipid peroxidation and oxidative damage of proteins occur in the microsomes of all the extrahepatic tissues including lung, kidney, adrenal gland and brain and that both the lipid peroxidation and protein damage are specifically prevented by ascorbic acid. We further demonstrate that when NADPH is replaced by\(O_2^{\bar \cdot } \) as the electron donor, the\(O_2^{\bar \cdot } \) lipid peroxidation and protein damage are also inhibited by ascorbic acid.

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Abbreviations

AH2 :

ascorbic acid

SOD:

bovine erythrocyte superoxide dismutase

GSH:

glutathione

XOD:

xanthine oxidase

cyt P450:

cytochrome P450

DFO:

desferrioxamine

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Authors and Affiliations

  1. Department of Biochemistry, University College of Science, 35 Ballygunge Circular Road, 700019, Calcutta, India

    Chinmay K. Mukhopadhyay, Mrinal K. Ghosh & Indu B. Chatterjee

  2. Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University College of Science, 35 Ballygunge Circular Road, 700019, Calcutta, India

    Chinmay K. Mukhopadhyay, Mrinal K. Ghosh & Indu B. Chatterjee

Authors
  1. Chinmay K. Mukhopadhyay
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  2. Mrinal K. Ghosh
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  3. Indu B. Chatterjee
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Mukhopadhyay, C.K., Ghosh, M.K. & Chatterjee, I.B. Ascorbic acid prevents lipid peroxidation and oxidative damage of proteins in guinea pig extrahepatic tissue microsomes. Mol Cell Biochem 142, 71–78 (1995). https://doi.org/10.1007/BF00928915

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  • DOI: https://doi.org/10.1007/BF00928915

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