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Menadione- and cyclic AMP-induced alteration of the ratio between D-[2-3H]glucose detritiation and phosphorylation in rat erythrocytes

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Abstract

When rat erythrocytes are exposed to both dibutyryl-cyclic AMP and isobutylmethylxanthine, the rates of D-glucose phosphorylation and glycolysis are both markedly increased. On the contrary, menadione by increasing the flow rate through the pentose phosphate pathway, decreases the generation rate of triose phosphates from exogenous D-glucose. Yet, both procedures lead to a decrease in the production of3HOH from D-[2-3H]glucose relative to its phosphorylation rate. It is proposed, therefore, that such a radioactive measurement should no more be considered as a reliable index of D-glucose phosphorylation, at variance with current practice.

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References

  1. Malaisse WJ, Yilmaz MT, Malaisse-Lagae F, Sener A: Underestimation of D-glucose phosphorylation as measured by3H2O production from D-[2-3H]glucose. Biochem Med Metab Biol 40: 35–41, 1988

    PubMed  Google Scholar 

  2. Rose IA, O'Connell EL: Intramolecular hydrogen transfer in the phosphoglucose isomerase reaction. J Biol Chem 236: 3086–3092, 1961

    PubMed  Google Scholar 

  3. Malaisse-Lagae F, Liemans V, Malaisse WJ: Phosphoglucoisomerase-catalyzed interconversion of hexosephosphates. Isotopic discrimination between hydrogen and tritium. Mol Cell Biochem 89: 57–67, 1989

    PubMed  Google Scholar 

  4. Liemans V, Malaisse-Lagae F, Willem R, Malaisse WJ: Phosphoglucoisomerase-catalyzed interconversion of hexose phosphates; diastereotopic specificity, isotopic discrimination and intramolecular hydrogen transfer. Biochim Biophys Acta 998: 111–117, 1989

    PubMed  Google Scholar 

  5. Malaisse WJ, Bodur H: Hexose metabolism in pancreatic islets: enzyme-to-enzyme tunnelling of hexose 6-phosphates. Int J Biochem 23: 1471–1481, 1991

    PubMed  Google Scholar 

  6. Liemans V, Malaisse WJ: Phosphoglucoisomerase-catalyzed interconversion of hexose phosphates: measurement of tritiated hexose phosphates in erythrocytes exposed to D-[2-3H]glucose. Med Sci Res 19: 427–428, 1991

    Google Scholar 

  7. Malaisse WJ: Altered ratio between D-[2-3H]glucose and D-[5-3H]glucose utilisation by erythrocytes of diabetic rats. Med Sci Res 18: 219–220, 1990

    Google Scholar 

  8. Malaisse WJ, De Vos A: Diabetes-induced alteration in D-[2-3H]glucose detritiation by erythrocytes: intrinsic or environmental perturbation of phosphoglucoisomerase? Med Sci Res 18: 305–306, 1990

    Google Scholar 

  9. Malaisse WJ: Environmental alteration of3HOH production from D-[1-3H]glucose, D-[2-3H]glucose and D-[5-3H]glucose by rat erythrocytes. Diab Res 15: 191–194, 1990

    Google Scholar 

  10. Manuel y Keenoy B, Zähner D, Malaisse WJ: Dissociated effects of 2-deoxy-D-glucose on D-[2-3H]glucose and D-[5-3H]glucose conversion to3HOH in rat erythrocytes. Biochem J 288: 433–438, 1992

    PubMed  Google Scholar 

  11. Manuel y Keenoy B, Malaisse-Lagae F, Malaisse WJ: Metabolism of tritiated D-glucose in rat erythrocytes. Metabolism 40: 978–985, 1991

    PubMed  Google Scholar 

  12. Malaisse WJ, Rasschaert J, Zähner D, Sener A: Hexose metabolism in pancreatic islets: the Pasteur effect. Diab Res 7: 53–58, 1988

    Google Scholar 

  13. Malaisse WJ, Sener A: Hexose metabolism in pancreatic islets. Feedback control of D-glucose oxidation by functional events. Biochim Biophys Acta 971: 246–254, 1988

    PubMed  Google Scholar 

  14. Malaisse WJ, Giroix M-H, Malaisse-Lagae F: 3-O-methyl-D-glucose transport in tumoral insulin-producing cells. Am J Physiol 251: C841-C846, 1986

    PubMed  Google Scholar 

  15. Sener A, Malaisse-Lagae F, Malaisse WJ: Fructose metabolism via the pentose cycle in tumoral islet cells. Eur J Biochem 170: 447–452, 1987

    PubMed  Google Scholar 

  16. Manuel y Keenoy B, Malaisse WJ: Assay of L-[3-3H]lactate generated from D-[1-3H]glucose, D-[2-3H]glucose and D-[6-3H]glucose in rat erythrocytes. Horm Metab Res 23: 395–396, 1991

    PubMed  Google Scholar 

  17. Passoneau JV: Fluorimetric method. In: HA Bergmeyer (ed) Methods of Enzymatic Analysis, Academic Press, San Diego, 1974, pp 1468–1472

    Google Scholar 

  18. Rose ZB, Liebowitz J: Direct determination of 2,3-diphosphoglycerate. Anal Biochem 35:177–180, 1970

    PubMed  Google Scholar 

  19. Ford DL, Omachi A: Influence of cyclic 3′,5′-AMP on glycolysis in human erythrocytes. Biochim Biophys Acta 279: 587–592, 1972

    PubMed  Google Scholar 

  20. Weisinger JR, Contreras NEIR, Cajias J, Bellorin-Font E, Amair P, Gutierrez L, Sylva V, Paz-Martinez V: Insulin binding and glycolytic activity in erythrocytes from dialyzed and non dialyzed uremic patients. Nephron 48: 190–196, 1988

    PubMed  Google Scholar 

  21. Katz J, Rognstad R: Metabolism of glucose-2-T by adipose tisue. J Biol Chem 244: 99–106, 1969

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Experimental Medicine, Brussels Free University, Erasmus Medical School, 808 Route de Lennik, CP 618, B-1070, Brussels, Belgium

    Begoña Manuel y Keenoy & Willy J. Malaisse

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  1. Begoña Manuel y Keenoy
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  2. Willy J. Malaisse
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Manuel y Keenoy, B., Malaisse, W.J. Menadione- and cyclic AMP-induced alteration of the ratio between D-[2-3H]glucose detritiation and phosphorylation in rat erythrocytes. Mol Cell Biochem 121, 5–11 (1993). https://doi.org/10.1007/BF00928694

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  • DOI: https://doi.org/10.1007/BF00928694

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