Abstract
A method of purifyingSarcocystis sporozoites following in vitro excystation is described. After passage of sporozoites through a modified DE 52 anion-exchange cellulose column, the mean recovery was 88% forS. capracanis, 71% forS hircicanis, 77% forS. ovicanis and 74% forS. arieticenis. Most of the sporozoites were motile, and the parasite suspension was free of excystation debris.
References
Bontemps M, Yvore P (1974) Technique de purification des suspensions de sporozoites d'Eimeria sur colonne de fibres synthetiques. Ann Rech Vet 5:109–113
Burgess DE, Speer CA, Reduker DW (1988) Identification of antigens ofSarcocystis cruzi sporozoites, merozoites, and bradyzoites with monoconal antibodies. J Parasitol 74:828–832
Heydorn AO, Haralambidis S, Matuschka FR (1981) Zur Chemoprophylaxe and Therapie der akuten Sarkosporidiose. Berl Münch Tierärztl Wochenschr 94:229–234
Horn K, Ono K, Heydorn AO (1991) In vitro excystation and cryopreservation of ovine and caprineSarcocystis species. J Protozool Res (in press)
Hosek JE, Todd KS, Kuhlenschmidt MS (1988) Improved method for high-yield excystation and purification of infective sporozoites ofEimeria spp. J Protozool 35:583–589
Lanham SM, Godfrey DG (1970) Isolation of salivarian trypanosomes from man and other mammals using. DEAE-cellulose Exp Parasitol 28:521–534
Larsen RA, Kyle JE, Whitmire WM, Speer CA (1984) Effects of nylon wool purification on infectivity and antigenicity ofEimeria falciformis sporozoites and merozoites. J Parasitol 70:597–601
Lumsden WHR, Kimber CD, Strange M (1977)Trypanosoma brucei: detection of low parasitaemia in mice by a miniature anion exchanger/centrifugation technique. Trans R Soc Trop Med Hyg 71:421–424
Mencke N (1989) Untersuchung von Sarkosporidien-Zystozoiten in der Immuno-Elektrophorese (Western Blot) und der Immuno-Elektronenmikroskopie mit Hilfe von monoklonalen Antikörpern gegenSarcocystis muris. Thesis, Veterinary School of Hannover
Reiter I, Mareis A (1986) Zur Differenzierung vonS. muris- undS. dispersa-Infektionen der Maus mittels Isoelektrofokussierung und Immunoassays. Dtsch Tierärztl Wochenschr 93:433–437
Sanft S (1990) In vitro Excystation und Lebendkonservierung vonSarkosporidien-Sporozoiten. Veterinary Thesis, Free University of Berlin
Schmatz DM, Crane MSJ, Murray PK (1984) Purification ofEimeria sporozoites by DE-52 anion-exchange chromatography. J Protozool 31:181–183
Sommer I, Mehlhorn H, Rüger W (1991) Biochemical and immunological characterization of major surface antigens ofSarcocystis muris andS. suicanis cyst merozoites. Parasitol Res 77:204–211
Speer CA, Dubey JP (1986) Vascular phase ofS. cruzi cultured in vitro. Can J Zool 64:209–211
Tenter AM, Johnson MR, Zimmerman GL (1989) Differentiation ofSarcocystis species in European sheep by isoelectric focusing. Parasitol Res 76:107–114
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ono, K., Horn, K. & Heydorn, A.O. Purification of in vitro excystedSarcocystis sporozoites by passage through a modified DE 52 anion-exchange column. Parasitol Res 77, 717–719 (1991). https://doi.org/10.1007/BF00928689
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00928689