Abstract
A new method for making whole-mount immunocytochemical preparations of thick-skinned tapeworms is described. The tough tegument of the worms, which is impermeable to primary and secondary antisera, is removed by placing live worms in distilled water for 5–8 h, during which period the tegument is shed. The worms are thereafter fixed and stained as whole specimens using fluorescence immunocytochemical techniques. Both the main nervous structures and the finest neuronal details are revealed by this technique. Several types of antisera combinations are used.Diphyllobothrium dendriticum andEubothrium crassum served as model organisms in this study.
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Gustafsson MKS (1985) Cestode neurotransmitters. Parasitol Today 1:72–75
Gustafsson MKS (1987) Immunocytochemical demonstration of neuropeptides and serotonin in the nervous system of adultSchistosoma mansoni. Parasitol Res, 74:168–174
Gustafsson MKS (1990) The cells of a cestode.Diphyllobothrium dendriticum as a model in cell biology. In: Gusafsson MKS, Reuter M (eds) The early brain. Proceedings, Symposium on Invertebrate Neurobiology. Acta Acad Aboensis Ser B 50(1):13–44
Gustafsson MKS, Reuter M (1990) Flatworms as model organisms In: Gustafsson MKS, Reuter M (eds) The early brain. Proceedings, Symposium on Invertebrate Neurobiology. Acta Acad Aboensis Ser B 50(7):5–12
Gustafsson MKS, Wikgren MC (1989) Development of immunoreactivity to the invertebrate neuropeptide small cardiac peptide B in the tapewormDiphyllobothrium dendriticum. Parasitol Res 75:396–400
Gustafsson MKS, Wikgren MC, Karhi TJ, Schot LPC (1985) Immunocytochemical demonstration of neuropeptides and serotonin in the tapewormDiphyllobothrium dendriticum. Cell Tissue Res 240:255–260
Gustafsson MKS, Lehtonen MAI, Sundler F (1988) Immunocytochemical evidence for the presence of “mammalian” neurohormonal peptides in neurones of the tapewormDiphyllobothrium dendriticum. Cell Tissue Res 243:41–49
Halton DW, Fairweather I, Shaw C, Johnston CF (1990) Regulatory peptides in parasitic platyhelminths. Parasitol Today 6:284–290
Johnston CF, Shaw C, Halton DW, Fairweather I (1990) Confocal scanning laser microscopy and helminth neuroanatomy. Parasitol Today 6:305–308
Maule AG, Halton DW, Johnston CF, Shaw C, Fairweather I (1990) The serotoninergic, cholinergic and peptidergic components of the nervous system of the monogenean parasite,Diclidophora merlangi: a cytochemical study. Parasitology 100:255–273
Reuter M (1987) Immunocytochemical demonstration of serotonin and neuropeptides in the nervous system ofGyrodactylus salaris (Monogenea) Acta Zool 68:187–193
Reuter M, Palmberg I (1989) Development and differentiation of neuronal subsets in asexually reproducingMicrostomum lineare. Immunocytochemistry of 5-HT, RF-amide and SCPR. Histochemistry 91:123–131
Reuter M, Wikgren M, Lehtonen M (1986) Immunocytochemical demonstration of 5-HT-like and FMRF-amide-like substances in whole mounts ofMicrostomum lineare (Turbellaria). Differences in distribution and development revealed by whole moum immunocytochemistry. Cell Tissue Res 24:7–12
Skuce PJ, Johnston CF, Fairweather I, Halton dW, Shaw C (1990) A confocal scanning laser microscope study of the peptidergic and serotoninergic components of the nervous system in larvalSchistosoma mansoni. Parasitology 101:227–234
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Gustafsson, M.K.S. Skin the tapeworms before you stain their nervous system!. Parasitol Res 77, 509–516 (1991). https://doi.org/10.1007/BF00928419
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DOI: https://doi.org/10.1007/BF00928419