Parasitology Research

, Volume 77, Issue 6, pp 509–516 | Cite as

Skin the tapeworms before you stain their nervous system!

A new method for whole-mount immunocytochemistry
  • Margaretha K. S. Gustafsson
Original Investigations

Abstract

A new method for making whole-mount immunocytochemical preparations of thick-skinned tapeworms is described. The tough tegument of the worms, which is impermeable to primary and secondary antisera, is removed by placing live worms in distilled water for 5–8 h, during which period the tegument is shed. The worms are thereafter fixed and stained as whole specimens using fluorescence immunocytochemical techniques. Both the main nervous structures and the finest neuronal details are revealed by this technique. Several types of antisera combinations are used.Diphyllobothrium dendriticum andEubothrium crassum served as model organisms in this study.

Keywords

Nervous System Model Organism Nervous Structure Immunocytochemical Technique Secondary Antiserum 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Gustafsson MKS (1985) Cestode neurotransmitters. Parasitol Today 1:72–75Google Scholar
  2. Gustafsson MKS (1987) Immunocytochemical demonstration of neuropeptides and serotonin in the nervous system of adultSchistosoma mansoni. Parasitol Res, 74:168–174Google Scholar
  3. Gustafsson MKS (1990) The cells of a cestode.Diphyllobothrium dendriticum as a model in cell biology. In: Gusafsson MKS, Reuter M (eds) The early brain. Proceedings, Symposium on Invertebrate Neurobiology. Acta Acad Aboensis Ser B 50(1):13–44Google Scholar
  4. Gustafsson MKS, Reuter M (1990) Flatworms as model organisms In: Gustafsson MKS, Reuter M (eds) The early brain. Proceedings, Symposium on Invertebrate Neurobiology. Acta Acad Aboensis Ser B 50(7):5–12Google Scholar
  5. Gustafsson MKS, Wikgren MC (1989) Development of immunoreactivity to the invertebrate neuropeptide small cardiac peptide B in the tapewormDiphyllobothrium dendriticum. Parasitol Res 75:396–400Google Scholar
  6. Gustafsson MKS, Wikgren MC, Karhi TJ, Schot LPC (1985) Immunocytochemical demonstration of neuropeptides and serotonin in the tapewormDiphyllobothrium dendriticum. Cell Tissue Res 240:255–260Google Scholar
  7. Gustafsson MKS, Lehtonen MAI, Sundler F (1988) Immunocytochemical evidence for the presence of “mammalian” neurohormonal peptides in neurones of the tapewormDiphyllobothrium dendriticum. Cell Tissue Res 243:41–49Google Scholar
  8. Halton DW, Fairweather I, Shaw C, Johnston CF (1990) Regulatory peptides in parasitic platyhelminths. Parasitol Today 6:284–290Google Scholar
  9. Johnston CF, Shaw C, Halton DW, Fairweather I (1990) Confocal scanning laser microscopy and helminth neuroanatomy. Parasitol Today 6:305–308Google Scholar
  10. Maule AG, Halton DW, Johnston CF, Shaw C, Fairweather I (1990) The serotoninergic, cholinergic and peptidergic components of the nervous system of the monogenean parasite,Diclidophora merlangi: a cytochemical study. Parasitology 100:255–273Google Scholar
  11. Reuter M (1987) Immunocytochemical demonstration of serotonin and neuropeptides in the nervous system ofGyrodactylus salaris (Monogenea) Acta Zool 68:187–193Google Scholar
  12. Reuter M, Palmberg I (1989) Development and differentiation of neuronal subsets in asexually reproducingMicrostomum lineare. Immunocytochemistry of 5-HT, RF-amide and SCPR. Histochemistry 91:123–131Google Scholar
  13. Reuter M, Wikgren M, Lehtonen M (1986) Immunocytochemical demonstration of 5-HT-like and FMRF-amide-like substances in whole mounts ofMicrostomum lineare (Turbellaria). Differences in distribution and development revealed by whole moum immunocytochemistry. Cell Tissue Res 24:7–12Google Scholar
  14. Skuce PJ, Johnston CF, Fairweather I, Halton dW, Shaw C (1990) A confocal scanning laser microscope study of the peptidergic and serotoninergic components of the nervous system in larvalSchistosoma mansoni. Parasitology 101:227–234Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Margaretha K. S. Gustafsson
    • 1
  1. 1.Department of BiologyAbo Akademi UniversityÅboFinland

Personalised recommendations