Abstract
An improved method for the purification ofTheileria parva schizonts from infected bovine cells is described. The technique is simpler and more rapid than previously described methods and gives rise to greater yields of schizonts with negligible contamination by host-cell components. In addition, a fluorescent staining technique was developed whereby live schizonts purified from infected cells can be enumerated and sorted using the flow cytometer. An assessment of the quality of schizonts prepared according to our method as a source of RNA for the construction of parasite cDNA libraries suggests that RNA derived from these preparations is free of host nucleic acids.
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Goddeeris, B.M., Dunlap, S., Innes, E.A. et al. A simple and efficient method for purifying and quantifying schizonts fromTheileria parva-infected cells. Parasitol Res 77, 482–484 (1991). https://doi.org/10.1007/BF00928414
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DOI: https://doi.org/10.1007/BF00928414
Keywords
- Nucleic Acid
- Infected Cell
- cDNA Library
- Efficient Method
- Flow Cytometer