Molecular and Cellular Biochemistry

, Volume 140, Issue 1, pp 37–48 | Cite as

Membrane associated proteoglycans in rat testicular peritubular cells

  • Lahcen Bichoualne
  • Bénédicte Thiébot
  • Monique Langris
  • Pierre Barbey
  • Hamid Oulhaj
  • Jean Bocquet


Confluent testicular peritubular cells derived from immature rats were used to study membrane associated proteoglycans (PG) Peripheral material (heparin releasable), membrane and intracellular material (Triton X-100 releasable) were collected, purified by anion exchange chromatography then characterized by gel filtration and by hydrophobic interaction chromatography, followed by enzymatic digestion and chemical treatment. The peripheral material was constituted of two populations of PG (Kav=0 and 0.10 on Superose 6 column), each containing both heparan sulfate proteoglycans (HSPG) and chondroitin proteoglycans (CSPG) and perhaps a hybrid PG (HSCSPG). These PG being not retained on an octyl Sepharose column they were devoided of hydrophobic properties. The integral membrane proteoglycans isolated on the basis of their hydrophobic properties represented 20% of the Triton X-100 releasable material, and were exclusively constituted of proteoheparan sulfate. There were no relationships between this membrane HSPG and the peripheral HSPG as evidenced by pulse chase experiments. The mode of intercalation of the hydrophobic HSPG in the cell membrane was studied. The majority of these macromolecules (80%) were sensitive to trypsin and only a minor proportion (20%) were sensitive to phosphatidylinositol specific phospholipase C. Thus, about 80% of the hydrophobic HSPG were intercalated in the cell membrane by a hydrophobic segment of the core protein whereas about 20% were associated with the cell membrane via a phosphatidylinositol residue covalently bound to the core protein of the PG.

Key words

heparan sulfate proteoglycans rat testicular peritubular cells membrane associated proteoglycans 





Chondroitin Sulfate Proteoglycans


Heparan Sulfate Proteoglycans


Heparan and Chondroitin Sulfate Proteoglycans


Deoxyribonuclease I


Dulbeccos modified Eagle's medium




Extracellular Matrix


Phosphate Buffered Saline




Glycosyl Phosphatidylinositol


Phosphatidylinositol Specific Phospholipase C


Tris Buffered Saline


Soybean Trypsin Inhibitor




Hyaluronic Acid


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Lahcen Bichoualne
    • 1
  • Bénédicte Thiébot
    • 1
  • Monique Langris
    • 1
  • Pierre Barbey
    • 1
  • Hamid Oulhaj
    • 1
  • Jean Bocquet
    • 1
  1. 1.Laboratoire de Biochimie, ER CNRS 90, IRBAUniversité de CaenCAEN CedexFrance

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