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Zeitschrift für Parasitenkunde

, Volume 71, Issue 3, pp 313–316 | Cite as

Maintenance of in vitro cultures ofBabesia divergens andBabesia major at low temperatures

  • J. Konrad
  • E. U. Canning
  • L. P. Phipps
  • J. Donnelly
Original Investigations

Abstract

Cultures of erythrocytes parasitized byBabesia divergens andBabesia major were stored in medium cooled to 4°C for up to 8 weeks. There was a marked decrease in parasitaemia and an increase in the number of free extra-erythrocytic, unagglutinated merozoites, during the cooled period. Cultures stored in this way and returned to 38°C resumed growth, with or without sub-culture. At the low temperature, only one sub-culture is required per week.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • J. Konrad
    • 1
  • E. U. Canning
    • 1
  • L. P. Phipps
    • 2
  • J. Donnelly
    • 2
  1. 1.Department of Pure and Applied BiologyImperial CollegeLondonUK
  2. 2.Ministry of Agriculture, Fisheries, and Food Department of ParasitologyCentral Veterinary LaboratoryWeybridgeUK

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