Abstract
TwoLeishmania donovani glycoconjugate ligands for the internalization receptor on BALB/c peritoneal macrophages [fucose-mannose ligand (FML) and phosphate mannogalactan ligand (PMGL)] were shown to be species-specific in a comparative phagocytosis-inhibition test. Promastigotes ofL. donovani Sudan (LD1S),L. infantum, L. d. donovani, L. major (Jericho and Sudan),L. tropica, L. chagasi, L. mexicana venezuelensis, L. m. mexicana, L. m. amazonensis, L. m. pifanoi, L. m. garnhami, L. braziliensis braziliensis, L. m. amazonensis (Josefa),L. enrietti orL. adleri were incubated with macrophages in the presence of 10 μg/ml FML and PMGL purified fromL. donovani (LD1S). Parasite internalization was determined and compared with that obtained in control experiments. Specific inhibition of phagocytosis ranged from 83% (L. donovani LD1S) to 7% (L. m. amazonensis). We could distinguish groups ofLeishmania consistently with their geographic distribution and the clinical aspects of the disease. Analogous experiments withL. m. amazonensis glycoconjugates showed reciprocal results, with inhibition ranging from 76% (L. m. amazonensis) to 8% (L. donovani LD1S).L. chagasi remained separated from the Old World kalaazar agents. Possible phylogenetic implications of these observations are discussed.
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Palatnik, C.B., Previato, J.O., Mendonça-Previato, L. et al. A new approach to the phylogeny ofLeishmania: species specificity of glycoconjugate ligands for promastigote internalization into murine macrophages. Parasitol Res 76, 289–293 (1990). https://doi.org/10.1007/BF00928181
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DOI: https://doi.org/10.1007/BF00928181