Zeitschrift für Parasitenkunde

, Volume 60, Issue 2, pp 185–192 | Cite as

Further observations on ultrastructural changes in hepatocytes of mice infected withSchistosoma mansoni

  • Linda G. Jones
  • Burton J. Bogitsh
Original Investigations

Summary

The effect of hepatic granulomas initiated by eggs ofSchistosoma mansoni on the ultrastructure of hepatocytes of murine hosts was studied. Specimens of infected livers were collected at half week intervals, starting at week 7 postinfection and terminating at week 9 postinfection. Only the hepatocytes adjacent to granulomas showed any alteration in structure. The most obvious change was the proliferation of smooth-surfaced endoplasmic reticulum, especially striking in samples collected 8 1/2 and 9 weeks postinfection. The hypothesis was presented that the material secreted by the egg of the organism might be responsible for what appeared to be a morphologic detoxification response by the hepatocytes. Other alterations evident in the hepatocytes were an increase in the lysosomal population, mitochondrial changes and a slight hypertrophy of the Golgi complexes. Previous related studies by other investigators were explored and the findings compared with the results of this study.

Keywords

Endoplasmic Reticulum Obvious Change Ultrastructural Change Golgi Complex Infected Liver 

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References

  1. Bloch, E.H., Wahab, M.F.A., Warren, K.S.: In vivo microscopic observations of the pathogenesis and pathophysiology of hepatosplenic schistosomiasis in the mouse liver. Am. J. Trop. Med. Hyg.21, 546–557 (1972)Google Scholar
  2. Bogitsh, B.J., Carter, C.E.: Immunocytochemical studies onSchistosoma mansoni. I. Soluble antigen in eggshell-enclosed miracidium. J. Parasitol.61, 1031–1040 (1975)Google Scholar
  3. Bogitsh, B.J., Wikel, S.K.:Schistosoma mansoni: ultrastructural observations on the small intestine of the murine host. Exp. Parasitol.35, 68–79 (1974)Google Scholar
  4. Boros, D.L., Warren, K.S.: Delayed hypersensitivity-type granuloma formation and dermal reaction induced and elicited by a soluble factor isolated fromSchistosoma mansoni eggs. J. Exp. Med.132, 488–507 (1970)Google Scholar
  5. Cardell, R.R.: Smooth endoplasmic reticulum in rat hepatocytes during glycogen deposition and depletion. In: International review of cytology, G.H. Bourne, J.F. Danielli, eds., Vol. 48, pp. 221–279. New York: Academic Press 1977Google Scholar
  6. Cha, Y.N., Edwards, R.: Effect ofSchistosoma mansoni infection on the hepatic drug-metabolizing capacity of mice. J. Pharmacol. Exp. Ther.199, 432–440 (1976)Google Scholar
  7. Hsu, S.Y.L., Hsu, H.F., Davis, J.R., Lust, G.L.: Comparative studies on the lesions caused by eggs ofSchistosoma japonicum andSchistosoma mansoni in livers of albino mice and rhesus monkeys. Ann. Trop. Med. Parasitol.66, 89–97 (1972)Google Scholar
  8. Jones, A.L., Fawcett, D.W.: Hypertrophy of the agranular endoplasmic reticulum in hamster liver induced by phenobarbital (with a review of the functions of this organelle in liver). J. Histochem. Cytochem.14, 215–232 (1966)Google Scholar
  9. Kuriyama, Y., Omura, T.: Effects of phenobarbital on the synthesis and degradation of the protein components of rat livers. Microsomal membranes. J. Biol. Chem.244, 2017–2026 (1969)Google Scholar
  10. Lichtenberg, F. von: Host response to eggs ofS. mansoni. I. Granuloma formation in the unsensitized laboratory mouse. Am. J. Pathol.41, 711–731 (1962)Google Scholar
  11. Lichtenberg, F. von: Studies on granuloma formation. III. Antigen sequestration and destruction in the schistosome pseudotubercle. Am. J. Pathol.45, 75–94 (1964)Google Scholar
  12. Lieber, C.S.: Quantification of the role of liver microsomes in ethanol metabolism after chronic alcohol consumption and associated alterations in drug and lipid metabolism. In: The liver. Quantitative aspects of structure and function. pp. 24–258. New York: Karger 1973Google Scholar
  13. Lumsden, R.D., Griffith, L.M.: Hepatic glycogen depletion in murineSchistosomiasis mansoni. Abstracts, 50th Annual Meeting American Society of Parasitology, p. 64, Allen Press, Lawrence, KS 1975Google Scholar
  14. Menard, D., Penasse, W., Drochmans, P., Hugon, J.S.: Glucose-6-phosphatase heterogenity within the hepatic lobule of the phenobarbital-treated rat. Histochemistry38, 299–329 (1974)Google Scholar
  15. Novikoff, A.B., Novikoff, P.M., Davis, C., Quintana, N.: Studies of microperoxisomes. II. A cytochemical method for light and electron microscopy. J. Histochem. Cytochem.20, 1006–1023 (1972)Google Scholar
  16. Popper, H., Schaffner, F.: Pathopsychology cholestasis. Hum. Pathol.I, 1–24 (1970)Google Scholar
  17. Ramadan, M.A.: Ultrastructure of hepatic parenchyma in murine hepatosplenic bilharziasis. Virchows Arch. [Cell Pathol.]9, 1–15 (1971)Google Scholar
  18. Reynolds, E.S., Ree, H., Moslen, M.: Liver parenchymal cell injury. IX. Phenobarbital potentiation of endoplasmic reticulum denaturation following carbon tetrachloride. Lab. Invest.26, 290–299 (1972)Google Scholar
  19. Stenger, R.J., Warren, K.S., Johnson, E.A.: An electron microscopic study of the liver parenchyma and schistosome pigment in murine hepatosplenic schistosomiasis mansoni. Am. J. Trop. Med. Hyg.16, 473–482 (1967)Google Scholar
  20. Warren, K.S.: The etiology of hepatosplenic schistosomiasis mansoni in mice. Am. J. Trop. Med. Hyg.10, 870–876 (1961)Google Scholar
  21. Warren, K.S., Domingo, E.O., Cowan, R.B.T.: Granuloma formation around schistosome eggs as a manifestation of delayed hypersensitivity. Am. J. Pathol.51, 735–756 (1976)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Linda G. Jones
    • 1
  • Burton J. Bogitsh
    • 1
  1. 1.Department of BiologyVanderbilt UniversityNashvilleUSA

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