Skip to main content
Log in

Glycosidases ofEntamoeba histolytica

  • Original Investigations
  • Published:
Zeitschrift für Parasitenkunde Aims and scope Submit manuscript

Abstract

Activities of glycosidases ofEntamoeba histolytica were determined in cell homogenates and growth medium at neutral and acidic pH. Most of the activities were low. In homogenates, significant activity was found for α-glucosidase, α-amylase, N-acetyl-neuraminate lyase, β-N-acetyl-D-glucosaminidase, and hyaluronidase. In the growth medium, only two of these enzymes, α-amylase and β-N-acetyl-D-glucosaminidase, were significantly active.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Aminoff D, Morgan WTJ, Watkins WM (1952) Studies in immunochemistry. 11. The action of dilute alkali on the N-acetyl-hexosamines and the specific blood-group mucoids. Biochem J 51:379–389

    Google Scholar 

  • Ashworth JM, Quance J (1972) Enzyme synthesis in myxamoebae of the cellular slime moldDictyostelium discoideum during growth in axenic culture. Biochem J 126:601–608

    Google Scholar 

  • Avila JL, Casanova MA, Avila A, Bretana A (1979) Acid and neutral hydrolases inTrypanosoma cruzi. J Protozool 26:304–311

    Google Scholar 

  • Baker WB, Buetow DE (1976) Hydrolytic enzymes ofEuglena gracilis: characterization and activity as a function of culture age and carbon deprivation. J Protozool 23:167–176

    Google Scholar 

  • Bowen SM, Griffiths AJ, Lloyd D (1969) Enzyme distribution in an amoeba during encystment. Biochem J 115:41–42

    Google Scholar 

  • Cantz M, Kresse H (1974) Sandhoff disease: defective glycosaminoglycan catabolism in cultured fibroblasts and its correction by β-N-acetylhexosaminidase. Europ J Biochem 47:581–590

    Google Scholar 

  • Carter J, Smith EE (1973) The substrate specificity of neutral α-glucosidase from rabbit muscle. Arch Biochem Biophys 155:82–94

    Google Scholar 

  • Comb DG, Roseman S (1960) The structure and enzymatic synthesis of N-acetylneuraminic acid. J Biol Chem 235:2529–2537

    Google Scholar 

  • Diamond LS (1968) Techniques of axenic cultivation ofEntamoeba histolytica Schaudinn, 1903, and ofE. histolytica-like amebae. J Parasitol 54:1047–1056

    Google Scholar 

  • Diamond LS, Harlow DR, Cunnick CC (1978) A new medium for the axenic cultivation ofEntamoeba histolytica and otherEntamoeba. Trans R Soc Trop Med Hyg 72:431–432

    Google Scholar 

  • El-Hashimi W, Pittman F (1970) Ultrastructure ofEntamoeba histolytica trophozoites obtained from the colon and from in vitro cultures. Am J Trop Med Hyg 19:215–226

    Google Scholar 

  • Griffin JL (1972) Human amebic dysentery. Electron microscopy ofEntamoeba histolytica contacting, ingesting and digesting inflammatory cells. Am J Trop Med Hyg 21:895–906

    Google Scholar 

  • Hoare CA (1952) The food habits ofEntamoeba histolytica in its commensal phase. Parasitology 42:43–47

    Google Scholar 

  • Hoskins LC (1977) Degradation of mucus glycoproteins in the gastrointestinal tract. In: Horowitz M, Pigman W (eds), The glycoconjugates I, Academic Press, New York, p 235–253

    Google Scholar 

  • Jarumilinta R, Maegraith BG (1960) Hyaluronidase activity in stock cultures ofEntamoeba histolytica. Ann Trop Med Parasitol 54:118–128

    Google Scholar 

  • Jarumilinta R, Maegraith BG (1969) Enzymes ofEntamoeba histolytica. Bull WHO 41:269–273

    Google Scholar 

  • Juniper K (1978) Amoebiasis. Clin Gastroenterol 7:3–29

    Google Scholar 

  • Loomis WFJ (1969) Acetylglucosaminidase, an early enzyme in the development ofDictyostelium discoideum. J Bacteriol 97:1149–1154

    Google Scholar 

  • Lourdes Muñoz M de, Calderón J, Rojkind M (1982) The collagenase ofEntamoeba histolytica. J Exp Med 155:42–51

    Google Scholar 

  • Lowry OH (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  • Lundblad G, Huldt G, Elander M, Lind J, Slettengren K (1981) β-n-acetylglucosaminidase fromEntamoeba histolytica. Comp Biochem Physiol B 68:71–76

    Google Scholar 

  • Lushbaugh WB, Kairalla AB, Hofbauer AF, Arnaud P, Cantey IR, Pittman FE (1981) Inhibition ofEntamoeba histolytica cytotoxin by alpha-1 antiprotease and alpha-2 macroglobulin. Am J Trop Med Hyg 30:575–584

    Google Scholar 

  • Mattern CFT, Keister DB (1977) Experimental amebiasis. II. Hepatic amebiasis in the newborn hamster. Am J Trop Med Hyg 26:402–411

    Google Scholar 

  • McCaul TF, Poston RN, Bird RG (1977)Entamoeba histolytica andEntamoeba invadens: Chromium release from labeled human liver cells in culture. Exp Parasitol 43:342–352

    Google Scholar 

  • McLaughlin J, Faubert G (1977) Partial purification and some properties of a neutral sulfhydryl and an acid proteinase fromEntamoeba histolytica. Can J Microbiol 23:420–425

    Google Scholar 

  • Morgan NA, Howells L, Cartledge TG, Lloyd D (1973) The acid-hydrolase-containing organells of eucaryotic microoganisms. In: Reid E (ed), Methological development in biochemistry, Vol 3, Wolfson Bioanalytical Centre, University of Surrey, p 219–232

  • Neufeld EF, Lim RW, Shapiro LJ (1975) Inherited disorders of lysosomal metabolism. Ann Rev Biochem 44:357–376

    Google Scholar 

  • Orozco E (1981) Virulencia y propiedades de superficie deEntamoeba histolytica. Thesis, Mexico City

  • Romanowska E, Watkins WM (1963) Fractionation of neuraminidases in extracts ofTrichomonas foetus. Biochem J 87:37P-38P

    Google Scholar 

  • Santer UV, Yee-Foon, J, Glick MC (1978) A rapid assay for neuraminidase. The detection of two differences in activity associated with virus transformation. Biochim Biophys Acta 523:435–442

    Google Scholar 

  • Skoza L, Mohos S (1976) Stable thiobarbituric acid chromophore with dimethyl sulphoxide. Application to sialic acid assay in analytical de-O-acetylation. Biochem J 159:457–462

    Google Scholar 

  • Steiger RF, van Hoof F, Bontemps J, Nyssens-Jadin M, Druetz JE (1979) Acid hydrolases of trypanosomatid flagellates. Acta Trop (Basel) 36:335–341

    Google Scholar 

  • Vaes G, Jacques P (1965) Studies on bone enzymes. The assay of acid hydrolases and other enzymes in bone tissue. Biochem J 97:380–388

    Google Scholar 

  • Verpoorte JA (1972) Purification of two β-N-acetyl-D-glucosaminidases from beef spleen. J Biol Chem 347:4787–4793

    Google Scholar 

  • Weissman B, Meyer K, Sampson P, Linker A (1954) Isolation of oligosaccharides enzymatically produced from hyaluronic acid. J Biol Chem 208:417–429

    Google Scholar 

  • Woollen ME, Turner P (1965) Plasma N-acetyl-β-glucosaminidase and β-glucuronidase in health and disease. Clin Chim Acta 12:671–683

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Trissl, D. Glycosidases ofEntamoeba histolytica . Z. Parasitenkd. 69, 291–298 (1983). https://doi.org/10.1007/BF00927870

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00927870

Keywords

Navigation