Fecundity ofLitomosoides carinii (Nematoda, Filarioidea) in vivo and in vitro
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Several parameters concerning the reproduction ofLitomosoides carinii were assessed using quantitatively infected cotton rats (Sigmodon hispidus). The course of embryogenesis from the fertilization of eggs to the delivery of the first microfilariae was observed by daily autopsies during prepatency. The duration of embryogenesis in vivo could thus be determined as 18±2 days. The contents of embryos in the uteri of female worms had been examined at various intervals. At the onset of patency 7–8 weeks p.i. the females were 71±6 mm long and on average contained 308×103 embryos/female, of which 19% were pathologically altered. In the middle of patency 16–20 weeks p.i. the females had grown up to 100±11 mm in length and now contained 509×103 embryos/female, 25% of them were pathologically altered, the others were normally developed. A positive correlation between the body length of a female worm and its number of embryos in utero was evident. Additionally the percentage of pathologically altered embryos was increased with respect to the age of the worms. The calculated fecundity of a femaleL. carinii in vivo of around 20×103 microfilariae/female per day had been confirmed with worms maintained in vitro. Three combinations of media and serum supplements were used and their influence on embryogenesis evaluated.
KeywordsSerum Supplement Pleural Cavity Female Worm Filarial Parasite Filarial Worm
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- Illgen B (1975) Erprobung von Medien zur Haltung vonLitomosoides carinii (Filaridae: Nematoda) in vitro. Thesis for Diploma in Biology, University of TübingenGoogle Scholar
- Kanda T, Tasaka S (1966) Studies on cotton rat filariasis using quantitative inoculation with larvae ofLitomosoides carinii (Travassos 1919). Jap J Parasitol 15:138–147Google Scholar
- Niemöller K (1971) Prüfung von Mel D auf embryotoxische Wirkung beiLitomosoides carinii (Nematoda, Filariidae). Doctoral Dissertation, University of TübingenGoogle Scholar
- Petranyi G, Mieth H (1972) Eine rationelle Methode zur quantitativen Infection adäquater Labortiere mitLitomosoides carinii. Z Tropenmed Parasit 23:2–9Google Scholar
- Schulz-Key H (1983) Observations on the reproductivity ofOnchocerca volvulus. Third International Symposium of Invertebrate Reproduction Tübingen, AbstractsGoogle Scholar
- Schulz-Key H, Jean B, Albiez EJ (1980) Investigations on femaleOnchocerca volvulus for the evaluation of drug trials. Tropenmed Parasit 31:34–40Google Scholar
- Weinstein PP, Sawyer TK (1961) Survival of adultDirofilaria uniformis in vitro and their production of microfilariae. J Parasitol 47, Suppl:23–24Google Scholar
- Wenk P, Neef U (1969) Die Regulation der Parasitämie bei Filarien (Litomosoides carinii, Nematoda, Filariidae). Zool Anz Suppl 33:92–95Google Scholar