Zeitschrift für Parasitenkunde

, Volume 72, Issue 1, pp 121–131 | Cite as

Fecundity ofLitomosoides carinii (Nematoda, Filarioidea) in vivo and in vitro

  • J. Mössinger
  • P. Wenk
Original Investigations


Several parameters concerning the reproduction ofLitomosoides carinii were assessed using quantitatively infected cotton rats (Sigmodon hispidus). The course of embryogenesis from the fertilization of eggs to the delivery of the first microfilariae was observed by daily autopsies during prepatency. The duration of embryogenesis in vivo could thus be determined as 18±2 days. The contents of embryos in the uteri of female worms had been examined at various intervals. At the onset of patency 7–8 weeks p.i. the females were 71±6 mm long and on average contained 308×103 embryos/female, of which 19% were pathologically altered. In the middle of patency 16–20 weeks p.i. the females had grown up to 100±11 mm in length and now contained 509×103 embryos/female, 25% of them were pathologically altered, the others were normally developed. A positive correlation between the body length of a female worm and its number of embryos in utero was evident. Additionally the percentage of pathologically altered embryos was increased with respect to the age of the worms. The calculated fecundity of a femaleL. carinii in vivo of around 20×103 microfilariae/female per day had been confirmed with worms maintained in vitro. Three combinations of media and serum supplements were used and their influence on embryogenesis evaluated.


Serum Supplement Pleural Cavity Female Worm Filarial Parasite Filarial Worm 
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  1. Engelbrecht F, Schulz-Key H (1984) Observations on adultOnchocerca volvulus maintained in vitro. Trans Roy Soc Trop Med Hyg 78:212–215CrossRefPubMedGoogle Scholar
  2. Franke ED, Weinstein PP (1983)Dipetalonema viteae (Nematoda: Firarioidea): Culture of third-stage larvae to young adults in vitro. Science 221:161–163CrossRefPubMedGoogle Scholar
  3. Franke ED, Weinstein PP (1984) In vitro cultivation ofDipetalonema viteae third-stage larvae: Effect of the gas phase. J Parasitol 70:493–498CrossRefPubMedGoogle Scholar
  4. Hawking F (1954) The reproductive system ofLitomosoides carinii, a filarial parasite of the cotton rat; III. The number of microfilariae produced. Ann Trop Med Parasitol 48:382–385CrossRefGoogle Scholar
  5. Illgen B (1975) Erprobung von Medien zur Haltung vonLitomosoides carinii (Filaridae: Nematoda) in vitro. Thesis for Diploma in Biology, University of TübingenGoogle Scholar
  6. Johnson MH, Orihel TC, Beaver PC (1974)Dipetalonema viteae in experimentally infected jird,Meriones unguiculatus. I. Insemination, development from egg to microfilariae, reinsemination, and longevity of mated and unmated worms. J Parasitol 60:302–309CrossRefPubMedGoogle Scholar
  7. Kanda T, Tasaka S (1966) Studies on cotton rat filariasis using quantitative inoculation with larvae ofLitomosoides carinii (Travassos 1919). Jap J Parasitol 15:138–147Google Scholar
  8. McFadzean JA, Smiles J (1956) Studies ofLitomosoides carinii by phase-contrast microscopy: the development of larvae. J Helminthol Vol XXX, 1:25–32CrossRefGoogle Scholar
  9. Miegeville P, Bouillard C, Marjolet M, Vermeil C (1981) Nouvelle Contribution à l'Etude deDipetalonema viteae Maintien en Survie des Adultes in vitro. Bull Soc Path Exot 74:207–216PubMedGoogle Scholar
  10. Niemöller K (1971) Prüfung von Mel D auf embryotoxische Wirkung beiLitomosoides carinii (Nematoda, Filariidae). Doctoral Dissertation, University of TübingenGoogle Scholar
  11. Petranyi G, Mieth H (1972) Eine rationelle Methode zur quantitativen Infection adäquater Labortiere mitLitomosoides carinii. Z Tropenmed Parasit 23:2–9Google Scholar
  12. Schulz-Key H (1983) Observations on the reproductivity ofOnchocerca volvulus. Third International Symposium of Invertebrate Reproduction Tübingen, AbstractsGoogle Scholar
  13. Schulz-Key H, Jean B, Albiez EJ (1980) Investigations on femaleOnchocerca volvulus for the evaluation of drug trials. Tropenmed Parasit 31:34–40Google Scholar
  14. Taylor AER (1960a) The spermatogenesis and embryology ofLitomosoides carinii andDirofilaria immitis. J Helmithol Vol XXXIV, 1/2:3–12CrossRefGoogle Scholar
  15. Taylor AER (1960b) Maintenance of filarial worms in vitro. Exp Parasitol 9:113–120CrossRefPubMedGoogle Scholar
  16. Webber WAF (1954) The reproductive system ofLitomosoides carinii, a filarial parasite of the cotton rat; I. Development of gonads and initial insemination. Ann Trop Med Parasitol 48:367–374CrossRefPubMedGoogle Scholar
  17. Weinstein PP, Sawyer TK (1961) Survival of adultDirofilaria uniformis in vitro and their production of microfilariae. J Parasitol 47, Suppl:23–24Google Scholar
  18. Wenk P, Neef U (1969) Die Regulation der Parasitämie bei Filarien (Litomosoides carinii, Nematoda, Filariidae). Zool Anz Suppl 33:92–95Google Scholar
  19. Wenk P, Illgen B, Seitz HM (1978) In vitro — Versuche mitLitomosoides carinii (Nematoda: Filarioidea); I. Haltung von adulten Weibchen und Mikrofilarien sowie Ausstoß von Mikrofilarien in verschiedenen Kulturmedien. Z Parasitenkd 55:63–72CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • J. Mössinger
    • 1
  • P. Wenk
    • 1
  1. 1.Tropenmedizinisches Institut der UniversitätTübingenFederal Republic of Germany

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