Zeitschrift für Parasitenkunde

, Volume 69, Issue 4, pp 473–482 | Cite as

The lysate from bovine erythrocytes infected withBabesia bovis

Analysis of antigens and a report on their immunogenicity when polymerized with glutaraldehyde
  • B. V. Goodger
  • I. G. Wright
  • D. J. Waltisbuhl
Original Investigations

Abstract

A group ofBabesia bovis antigens obtained by a lengthy biochemical procedure involving disruption of infected erythrocytes has previously been shown to be highly protective. This study shows that these antigens can be found in a simple lysate of infected erythrocytes. The antigens have been characterized by gel filtration and nitrocellulose transfer and consist of a wide spectrum of molecular sizes. Some of the antigens exist in complex form and are easily dissociated. The lysate was polymerized with glutaraldehyde and injected per se into four splenectomized calves. All the calves produced antibody toB. bovis but did not produce erythrocytic isoantibodies. The vaccinated calves and a control group of four splenectomized calves were challenged with virulentB. bovis. Statistically, the vaccinated group differed significantly in parasitaemia, temperature change and pathophysiological parameters from the control group. All of the control group died whereas two of the vaccinated group survived infection.

Keywords

Temperature Change Complex Form Glutaraldehyde Wide Spectrum Molecular Size 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • B. V. Goodger
    • 1
  • I. G. Wright
    • 1
  • D. J. Waltisbuhl
    • 1
  1. 1.Division of Tropical Animal Science, Long Pocket LaboratoriesCSIROIndooroopillyAustralia

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