Advertisement

Zeitschrift für Parasitenkunde

, Volume 67, Issue 3, pp 279–287 | Cite as

Enzyme-linked immunosorbent assay with egg antigens ofSchistosoma japonicum

  • A. Ishii
  • M. Owhashi
Original Investigations

Abstract

A micromethod of the enzyme-linked immunosorbent assay (ELISA) was applied to infections withSchistosoma japonicum in humans and mice with egg antigens. In mouse infections, antibody responses were detected between 6 and 8 weeks after the cercarial challenge. In human infections, a circum oval precipitin positive group showed the highest reaction values in ELISA. ELISA with egg antigens did not show any cross reaction with sera of humans infected with intestinal parasites (Ascaris, Trichuris and hookworms). Fractionation of the egg antigen was attempted using gel filtration, ion-exchange and lectin affinity chromatographies. Antigenic fractions were detected in two molecular weight groups of glycoprotein, which showed major pI value at 5–6. However, ELISA with partially purified antigen (JEA-1) did not give better sensitivity or specificity than the crude extract.

Keywords

Crude Extract Affinity Chromatography Mouse Infection Cross Reaction Human Infection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Carter CE, Colley DG (1981)Schistosoma japonicum soluble egg antigens: Separation by ConA chromatography and immunoaffinity purification. Mol Immunol 18:219–225PubMedGoogle Scholar
  2. Garcia EG, Cabrera BD, Cristi ZA, Silan RB (1968) Evaluation of some laboratory procedures for the diagnosis of schistosomiasis. Acta Med Philippina 4:130–137Google Scholar
  3. Hillyer GV, Gomez de Rios (1979a) The enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of schistosomiasis. Am J Trop Med Hyg 28:237–241PubMedGoogle Scholar
  4. Hillyer GV, Tiben ER, Knight WB, Gomez de Rios I, Pelley RP (1979b) Immunodiagnosis of infection withSchistosoma mansoni; Comparison of ELISA, radioimmunoassay, and precipitation tests performed with antigens from eggs. Am J Trop Med Hyg 28:661–669PubMedGoogle Scholar
  5. Ishii A, Owhashi M, Hashiguchi J (1982)Schistosoma japonicum: Immediate hypersensitivity reactions to egg antigen in man and rodents. Exp Parasitol, in pressGoogle Scholar
  6. Kabasawa Y, Ishii A (1979) Studies on the physicochemical properties of house dust mite (Dermatophagoides pteronyssinus) allergens involved in reaginic reaction. Jap J Exp Med 49:51–57PubMedGoogle Scholar
  7. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  8. Lunde MN, Ottesen EA (1980) Enzyme-linked immunosorbent assay (ELISA) for detecting IgM and IgE antibodies in human schistosomiasis. Am J Trop Med Hyg 29:82–85PubMedGoogle Scholar
  9. Matsuda H, Nakao M, Tanaka H, Blas BL, Nosenas JS (1980) Application of micro-ELISA to the sero-epidemiological survey of schistosomiasis japonica. Jap J Parasit, suppl 30Google Scholar
  10. McLaren ML, Lillywhite JE, Dunne DW, Doenhoff MJ (1981 Serodiagnosis of humanSchistosoma mansoni infections: enhanced sensitivity and specificity in ELISA using a fraction containingS. mansoni egg antigens w1 and a1. Trans Roy Soc Trop Med Hyg 75:72–79PubMedGoogle Scholar
  11. McLaren ML, Long EG, Goodgame RW, Lillywhite JE (1979) Application of the enzyme immunosorbent assay (ELISA) for the serodiagnosis ofSchistosoma mansoni infection in St. Lucia. Trans Roy Soc Trop Med Hyg 73:636–639PubMedGoogle Scholar
  12. Owhashi M, Ishii A (1981) Fractionation and characterization of allergens extracted from eggs ofSchistosoma japonicum. Int Arch Allergy appl. Immunol 64:146–156PubMedGoogle Scholar
  13. Tanaka H, Matsuda H, Nosenas JS (1979) Detection of antibodies inSchistosoma japonicum infections by a microtechnique of enzyme-linked immunosorbent assay (ELISA). Jap J Exp Med 49:289–292PubMedGoogle Scholar
  14. Voller A, Bartlett A, Bidwell DE (1976a) Enzyme immunoassays for parasitic diseases. Trans Roy Soc Trop Med Hyg 70:98–106PubMedGoogle Scholar
  15. Voller A, Bidwell DE, Bartlett A (1976b) Enzyme immunoassays in diagnostic medicine, theory and practice. Bull Wld Health Org 53:55–65Google Scholar
  16. Warren KS, Grove DI, Pelley RP (1978) TheSchistosoma japonicum egg granuloma. II. Cellular composition, granuloma size, and immunologic concomitants. Am J Trop Med Hyg 27:271–275PubMedGoogle Scholar
  17. Westerberg O (1971) Isoelectric focusing of proteins. Methods in Enzymology 22:389–412Google Scholar
  18. Yokogawa M, Sano M, Araki K (1967) Immunodiagnosis of schistosomiasis japonica. III. Circumoval precipitation test. Jap J Parasit 16:77–84Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • A. Ishii
    • 1
  • M. Owhashi
    • 1
  1. 1.Department of ParasitologyMiyazaki Medical CollegeMiyazakiJapan

Personalised recommendations