Abstract
A simple technique for obtaining ultra-thin sections of coccidian oocysts is reported. Oocyst walls, the main barriers to fixatives, dehydrating agents and embedding media are ruptured by brief immersion of the organism in liquid nitrogen. Adequate penetration of chemicals is thus assured but with minimal damage to the internal tissue. The fine structure of sporulation of oocysts ofEimeria tenella was studied using this process. Each oocyst produced four sporoblasts. These developed into sporocysts and each produced two sporozoites.
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References
Birch-Andersen A, Ferguson DJP, Pontefract RD (1976) A technique for obtaining thin sections of coccidian oocysts. Acta Path Microbiol Scand. Sect B 84:235–239
Ferguson DJP, Birch-Andersen A, Hutchison WM, Siim JC (1978a) Light and electron microscopy on the sporulation of the oocysts ofEimeria brunetti I. Development of the Zygote and Formation of the Sporoblasts. Acta Path Microbiol Scand. Sect B 86:1–11
Ferguson DJP, Birch-Andersen A, Hutchison WM, Siim JC (1978b) Light and electron microscopy on the sporulation of the oocysts ofEimeria brunetti II. Development into the sporocyst and formation of the sporozoite. Acta Path Microbiol Scand. Sect B 86:13–24
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Beesley, J.E., Latter, V.S. The sporulation ofEimeria tenella as revealed by a novel preparative method. Z. Parasitenkd. 67, 255–260 (1982). https://doi.org/10.1007/BF00927660
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DOI: https://doi.org/10.1007/BF00927660