Zeitschrift für Parasitenkunde

, Volume 59, Issue 3, pp 235–243 | Cite as

Evaluation of lung recovery assay for schistosomula in mice immunized with X-irradiated cercariae ofSchistosoma mansoni

  • S. Y. Li Hsü
  • H. F. Hsü
  • L. F. Burmeister
  • J. W. Osborne
Article

Summary

The lung recovery assay for schistosomula has recently been used as a rapid method for measuring the state of immunity of a challenged animal. Its merit rests on the fact that the peak day of schistosomular recovery in the lungs of both normal and immune hosts falls on the same day and the degree of immunity can be measured by the percent of schistosomular recovery on this particular day. An evaluation of this method for mice immunized with highly X-irradiated cercariae ofSchistosoma mansoni was made. The results indicate that in the immunized mice the peak day for the schistosomular recovery was delayed and the number of schistosomula recovered on the peak day was decreased. The delay of the peak day was increased by the degree of host immunity, so its identification for a host of an unknown immunologic status was not a simple matter. In addition, this method measured only the immune effect which took place in the skin and on prepeak days in the lungs. It was not as competent as the conventional liver perfusion method which measures the total result of the immune effect of the skin, lungs, and liver. Our conclusion is that for measuring the immunizing effect of irradiated cercariae, the lung recovery method is not suitable for the determination of the state of immunity induced.

Keywords

Rapid Method Immunize Mouse Simple Matter Host Immunity Recovery Method 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Armitage, P.: Statistical methods in medical research. 129–131. New York: John Wiley 1971Google Scholar
  2. Bancroft, T.A.: Intermediate statistical methods. 100–113. Ames, Iowa: Iowa State University Press 1968Google Scholar
  3. Hsü, S.Y.L., Davis, J.R., Hsü, H.F.: Pathology in rhesus monkeys infected with the Formosan strain ofSchistosoma japonicum. Z. Tropenmed. Parasitol.13, 341–356 (1962)Google Scholar
  4. Hsü, S.Y.L., Hsü, H.F., Penick, G.D., Lust G.L., Osborne, J.W., Cheng, H.F.: Mechanism of immunity to schistosomiases: Histopathological study of lesions elicited in rhesus monkeys during immunization and challenge with cercariae ofSchistosoma japonicum. J. Reticuloendothel. Soc.18, 167–185 (1975)Google Scholar
  5. Lewis, F.A., Colley, D.G.: Modification of the lung recovery assay for schistosomula and correlation with worm burdens in mice infected withSchistosoma mansoni. J. Parasitol.63, 413–417 (1977)Google Scholar
  6. Mahmoud, A.A.F., Warren, K.S., Peters, P.A.: Role for the eosinophil in acquired resistance toSchistosoma mansoni infection as determined by antieosinophil serum. J. Exp. Med.142, 805–813 (1975)Google Scholar
  7. Minard, P., Dean, D.A., Vannier, W.E., Murrel, K.D.: Effect of immunization on migration ofSchistosoma mansoni through lungs. Am. J. Trop. Med. Hyg.27, 87–93 (1978)Google Scholar
  8. Perez, H., Clegg, J.A., Smithers, S.R.: Acquired immunity toSchistosoma mansoni in the rat: measurement of immunity by the lung recovery technique. Parasitology69, 349–359 (1974)Google Scholar
  9. Sher, A., Mackenzie, P., Smithers, S.R.: Decreased recovery of invading parasites from the lungs as a parameter of acquired immunity to schistosomiasis in the mouse. J. Infect. Dis.130, 626–633 (1974)Google Scholar
  10. Snedecor, G.W., Cochran, W.G.: Statistical methods. 252–298. Ames, Iowa: Iowa State University Press 1967Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • S. Y. Li Hsü
    • 1
  • H. F. Hsü
    • 1
  • L. F. Burmeister
    • 1
  • J. W. Osborne
    • 1
  1. 1.Department of Preventive Medicine and Environmental Health and Radiation Research LaboratoryUniversity of Iowa College of MedicineIowa CityUSA

Personalised recommendations