Zeitschrift für Parasitenkunde

, Volume 57, Issue 2, pp 163–168 | Cite as

The use of deep frozen, stored bovine blood for in vitro feeding of tsetse flies

  • H. Wetzel
  • D. Luger


A method of blood preparation is described which resulted in the successful rearing ofGlossina p. palpalis with in vitro feeding. Cells of defibrinated bovine blood were washed in 0.89% NaCl solution prior to deep-freezing. Cells and serum were stored (−28°C) for up to four months. For feeding, both components were mixed in the volumetric proportion 1:1. Adenosine triphosphate was added at a concentration of 10−3 M to stimulate uptake of blood. Survival rate and mean weight of puparia remained constant over three generations while productivity increased.


Survival Rate Adenosine Triphosphate Adenosine Triphosphate Bovine Blood 
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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • H. Wetzel
    • 1
  • D. Luger
    • 1
  1. 1.Laboratory Joint FAO/IAEA DivisionViennaAustria

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