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Failure of short term stimulation to reduce sarcoplasmic reticulum Ca2+-ATPase function in homogenates of rat gastrocnemius

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To examine the effect of short term intense activity on sarcoplasmic reticulum (SR) Ca2+ sequestering function, the gastrocnemius (G) muscles of 11 anaesthetized male rats (weight, 411±8 g,X±SE) were activated using supramaximal, intermittent stimulation (one train of 0.2 msec impulses per sec of 100 msec at 100 Hz). Homogenates were obtained from stimulated white (WG-S) and red (RG-S) tissues, assayed for Ca2+ uptake and maximal Ca2+ ATPase activity and compared to contralateral controls (WG-C, RG-C). Calcium uptake (nmoles/mg protein/min) determined using Indo-l and at [Ca2+]f concentrations between 300–400 nM was unaffected (p>0.05) by activity in both WG (6.14+0.43 vs 5.37+0.43) and RG (3.21+0.18 vs 3.07+0.20). Similarly, no effect (p>0.05) of contractile activity was found for maximal Ca2+ ATPase activity (μmole/mg protein/min) determined spectrophotometrically in RG (0.276+0.03 vs 0.278+0.02). In WG, Ca2+ ATPase activity was 15% higher in WG-S compared to WG-C (0.412+0.03 vs 0.385+0.04). Repetitive stimulation resulted in a reduction in tetanic tension of 74% (p<0.05) by 2 min in the G muscle. By the end of the stimulation period, ATP concentration was reduced (p<0.05) by 57% in the WG and by 47% in the RG. These results indicate that the repeated generation of maximal tetanic force, at least for short term periods, need not adversely affectin vitro homogenate determination of Ca2+ sequestering function in spite of severe alterations in energy potential and that some other mechanism must be involved to explain the depression in Ca2+ uptake and Ca2+ ATPase activity previously noted with short term intense exercise.

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Authors and Affiliations

  1. Department of Kinesiology, University of Waterloo, N2L 3CI, Waterloo, Ontario, Canada

    J. Dossett-Mercer, H. Green, E. R. Chin & F. Grange

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  1. J. Dossett-Mercer
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  2. H. Green
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  3. E. R. Chin
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  4. F. Grange
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Dossett-Mercer, J., Green, H., Chin, E.R. et al. Failure of short term stimulation to reduce sarcoplasmic reticulum Ca2+-ATPase function in homogenates of rat gastrocnemius. Mol Cell Biochem 146, 23–33 (1995). https://doi.org/10.1007/BF00926877

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  • DOI: https://doi.org/10.1007/BF00926877

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