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Heat shock protein 72 in cardiac and skeletal muscles during hypertension

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Abstract

In order to elucidate the relationship between hypertension and hypertrophy in the production of heat shock proteins, we studied the induction of the HSP72 synthesis by the heart and gracilis muscles of normo (WKY) and hypertensive (SHR) rats subjected to hyperthermia (42°C±0.5 for 15 min). Two age groups were investigated in each strain: young (2 months, with developing cardiac hypertrophy) and old (18 months, with fully developed chronic cardiac hypertrophy). The gracilis muscle never developed hypertrophy, independently of hypertension or aging. 72 kDa inducible protein was determined by Western blot analysis using a specific monoclonal antibody. We also used a commercial standard, loaded on each blot, to quantitate densitometrically the signal.

The heart of young SHR responds to heat shock more than their normotensive age-matched control (298.8±24.7% vs 88.3 ±8.5%, p<0.001). This response is not maintained during aging as we did not find any significant difference between normo-and hypertensive old rats after exposure to hyperthermia (43.6±5.3% vs 65.3±10.4%).

Unlike the heart, the gracilis muscle shows a basal spontaneous HSP72 synthesis in both the SHR (71.4±10.8%) and WKY (40.6±11.7%) animals. There was a significant increase in HSP72 synthesis in the gracilis muscle of young SHR with respect to their control (186.2±18.7% vs 115.8±9.9%, p<0.02) which was maintained also during aging (171.9±17.3% vs 95.2±10.5%, p<0.01).

In conclusion, these data show that hypertension results in an increased synthesis of HSP72 both in cardiac and gracilis muscle in response to heat shock. This abnormal response is attenuated by aging in the heart but not in the gracilis muscle. Thus, the abnormality seems to be independent from hypertrophy and linked to genetic determination of the disease.

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References

  1. Currie RW, White FP: Characterization of the synthesis and accumulation of a 71 kDa protein induced in rat tissues after hyperthermia. Can J Biochem Cell Biol 61: 438–446, 1983

    Google Scholar 

  2. Currie RW: Effects of ischemia and perfusion temperature on the synthesis of stress-induced (heat-shock) proteins in isolated and perfused rat hearts. Mol Cell Cardiol 19: 795–808, 1987

    Google Scholar 

  3. Currie RW: Protein synthesis in perfused rat hearts afterin vivo hyperthermia andin vitro cold ischemia. Biochem Cell Biol 66: 13–19, 1988

    Google Scholar 

  4. Mehta HB, Popovich BK, Dillmann WH: Ischemia induces changes in the level mRNA coding for stress protein 71 and creatine kinase M. Circ Res 63: 512–517, 1988

    Google Scholar 

  5. Currie RW, Karmazyn M, Kloc M, Mailer K: Heat-shock response is associated with enhanced postischemic ventricular recovery. Circ Res 63: 543–549, 1988

    Google Scholar 

  6. Currie RW, Karmazyn M: Improved post-ischemic ventricular recovery in the absence of changes in energy metabolism in working rat hearts following heat shock. J Mol Cell Cardiol 22: 631–636, 1990

    Google Scholar 

  7. Yellon DM, Pasini E, Cargnoni A, Marber MS, Latchaman DS, Ferrari R: The protective role of heat stress in the ischemic and reperfused rabbit myocardium. J Mol Cell Cardiol 24: 895–907, 1992

    Google Scholar 

  8. Dillmann WH, Chi SH, Sayen MR, Mestril R: Decreased ischemia (Ism) induced injury in the rat heart derived H9c2 cells expressing human inducible heat shock protein 70 (hsp 70i). (Abstract) J Mol Cell Cardiol 24 (suppl III): S30, 1992

    Google Scholar 

  9. Howard G, Georghegan TE: Altered cardiac tissue gene expression during acute hypoxic exposure. Mol Cell Biochem 69: 155–160, 1986

    Google Scholar 

  10. Aldashev AA, Agibetov KA, Yugai AA, Shamshiev AT: Specific proteins synthesized in human lymphocytes during hypoxia. Am J Physiol 261/4 suppl.: 92–96, 1991

    Google Scholar 

  11. Lai YK, Havre PA, Hammond GL: Cardiac hypertrophy cannot proceed without initial suppression of protein synthesis. Biochem Biophys Res Commun 135/3: 857–863, 1986

    Google Scholar 

  12. Batist G, Merseareau W, Malashenko BA, Chiu RCJ: Response to ischemia-reperfusion injury in hypertrophic heart, role of free-radical metabolic pathway. Circulation 80(suppl 3): III-10–III-13, 1989

    Google Scholar 

  13. Li GC: Induction of thermotolerance and enhanced heat shock protein synthesis in Chinese hamster fibroblasts by sodium arsenite and by ethanol. J Cell Physiol 115: 116–122, 1983

    Google Scholar 

  14. Malo D, Tremblay J, Hamet P: Genetic and molecular characteristics of thermosensitivity in hypertension. J Hypertens 6(suppl 4): S55–57, 1988

    Google Scholar 

  15. Hamet P, Malo D, Tremblay J: Increased transcription of a major stress gene in spontaneously hypertensive mice. Hypertension 15: 904–908, 1990

    Google Scholar 

  16. Hamet P, Tremblay J, Pang SC, Walter SV, Wen YI: Primary versus second event in hypertension. Can J Physiol Pharmacol 63: 380–385 1985

    Google Scholar 

  17. Dubowitz V, Sewry AC: In: Muscle biopsy. A practical approach. Baillere Tindall, (ed.). 1986, pp 34–37

  18. Bradford MM: A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254, 1976

    Google Scholar 

  19. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 277: 680–685, 1970

    Google Scholar 

  20. Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrilamide gels to nitrocellulose sheets: procedure and some application. Proc Natl Acad Sci, USA 76: 4350–4354, 1979

    Google Scholar 

  21. Williams RR, Hunt SC, Hopkins PN, Laragh JK, Brenner BM: In: Hypertension: Pathophysiology, Diagnosis and Management. (eds) Raven Press Ltd, NY, 1990 p 127

    Google Scholar 

  22. Wurst W, Benesch C, Drabent B, Rothermel E, Benecke BB, Gunther E: Localization of heat shock protein 70 genes inside the rat major hystocompatibility complex close to class III genes. Immunogenetics 30: 46–49, 1989

    Google Scholar 

  23. Hamet P, Tremblay J, Malo D, Kunes J, Hashimoto T: Genetics hypertension is characterized by abnormal expression of a gene localized in major hystocompatibility complex HSP70. Transplantation Proceedings, 22(6): 2566–2567, 1990

    Google Scholar 

  24. Malo D, Tremblay J, Pang SC, Schlager G, Hamet P: Heat stress proteins in hypertension. Fed Proc 45: 899, 1986

    Google Scholar 

  25. Hamet P, Malo D, Hashimoto T, Tremblay J: Heat stress genes in hypertension. J Hypertens 8(suppl 7): S47–S52, 1990

    Google Scholar 

  26. Delcaire C, Samuel JL, Marotte F, Best-Belpomme M, Mercadier JJ, Rappaport LJ: Synthesis of stress proteins in rat cardiac myocytes 2–4 days after imposition of hemodinamic overload. J Clin Invest 82/2: 460–8, 1988

    Google Scholar 

  27. Chevalier B, Callens F, Charlemagne D, Delcayre C, Lompre AM, Lilievre L, Mercadier JJ, Moalic JM, Mansier P, Rappaport L, Samuel JL, Schwartz K, Swyinghedauw B: Signal and adaptational changes in gene expression during cardiac overload. J Mol Cell Cardiol 21(suppl 5): 71–77, 1989

    Google Scholar 

  28. Izumo S, Nadal-Ginard B, Mahdavi V: Protooncogene induction and reprogramming of cardiac gene expression produced by pressure overload. Proc Natl Acad Sci USA 85: 339–343, 1988

    Google Scholar 

  29. Fargnoli J, Kunisada T, Fornace AJ Jr, Schneider EL, Holbrook NJ: Decreased expression of heat shock protein 70 mRNA and protein after treatment in cells of aged rats. Proc Natl Acad Sci USA 87: 846–850, 1990

    Google Scholar 

  30. Blake MJ, Udelsman R, Feulnerg GJ, Norton DD, Holbrook NJ: Stress-induced heat shock protein 70 expression in adrenal cortex: an adrenocorticotropic hormone-sensitive, age-dependent response. Proc Natl Acad Sci USA 88: 9873–9877, 1991

    Google Scholar 

  31. Udelsman R, Blake MJ, Stagg CA, Li DG, Putney DJ, Holbrook NJ: Vascular heat shock protein expression in response to stress. Endocrine and automatic regulation of this age-dependent response. J Clin Invest 91: 465–473, 1993

    Google Scholar 

  32. Locke M, Noble EG, Atkinson BG: Inducible isoform of HSP70 is constitutively expressed in a muscle fiber type specific pattern. Am J Physiol 262: C774–779, 1991

    Google Scholar 

  33. Christie KN, Stoward PJ: Catalase in skeletal muscle fibers. J Histochem. Cytochem. 27: 814–819, 1979

    Google Scholar 

  34. Riley DA, Bain JLW, Ellis S, Haas L: Quantification and immunocytochemical localization of ubiquitin conjugates within rat red and white skeletal muscle. J Histochem Cytochem 36: 621–632, 1988

    Google Scholar 

  35. Obinata T, Maruyama K, Sugita H, Kohama K, Ebashi S: Dynamics aspects of structural proteins in vertebrate skeletal muscle. Muscle Nerve 4: 456–488, 1981

    Google Scholar 

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Authors and Affiliations

  1. Fondazione Clinica del Lavoro, Centro di Fisiopatologia Cardiovascolare ‘S. Maugeri’, Via Pinidolo, 23, 25064, Gussago, Brescia, Italy

    G. Gaia, L. Comini & E. Pasini

  2. Istituto di Clinica Neurologica, Universita' degli Studi di Verona, Italy

    G. Tomelleri

  3. Cattedra di Cardiologia, Universita' degli Studi di Brescia, Brescia, Italy

    L. Agnoletti & R. Ferrari

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  1. G. Gaia
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  2. L. Comini
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  3. E. Pasini
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  4. G. Tomelleri
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  5. L. Agnoletti
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  6. R. Ferrari
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Gaia, G., Comini, L., Pasini, E. et al. Heat shock protein 72 in cardiac and skeletal muscles during hypertension. Mol Cell Biochem 146, 1–6 (1995). https://doi.org/10.1007/BF00926874

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  • DOI: https://doi.org/10.1007/BF00926874

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