Molecular and Cellular Biochemistry

, Volume 119, Issue 1–2, pp 203–207 | Cite as

Purification and properties of pyridoxal kinase from bovine brain

  • Tae Hirakawa-Sakurai
  • Kiyoshi Ohkawa
  • Makoto Matsuda
Cellular Function and Metabolism

Abstract

A 27,000-fold purification of pyridoxal kinase from bovine brain tissue has been achieved by a combination of ammonium sulfate fractionation, DEAE-cellulose chromatography, hydroxyapatite chromatography, Sephadex G-150 gel filtration, Blue Sepharose CL-6B chromatography, and Phenyl-Superose chromatography. The final chromatography step yields a homogeneous preparation of high specific activity (2105 nmol/min/mg protein). The molecular mass of the native enzyme was estimated to be approximately 80,000 on gel filtration. The subunit molecular mass was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis to be approximately 39,500. This indicates that pyridoxal kinase is a dimeric enzyme.

Key words

pyridoxal kinase bovine brain vitamin B6 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Tae Hirakawa-Sakurai
    • 1
  • Kiyoshi Ohkawa
    • 1
  • Makoto Matsuda
    • 1
  1. 1.Department of BiochemistryJikei University School of MedicineTokyo 105Japan

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