Molecular and Cellular Biochemistry

, Volume 144, Issue 1, pp 67–74 | Cite as

Oxidative stress adaptation improves postischemic ventricular recovery

  • Nilanjana Maulik
  • Masazumi Watanabe
  • Daniel T. Engelman
  • Richard M. Engelman
  • Dipak K. Das


Adaptation to various forms of stress has been found to be associated with increased cellular tolerance to myocardial ischemia. In this study, the effects of myocardial adaptation to oxidative stress was examined by injecting rats with endotoxin (0.5 mg/kg) and its non-toxic derivative, lipid A (0.5 mg/kg). Both compounds exerted oxidative stress within 1 h of treatment as evidenced by enhanced malonaldehyde formation. The oxidative stress disappeared steadily and progressively with time in concert with the appearance of the induction of glutathione and antioxidative enzymes that included superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase. After 24 h of endotoxin or lipid A treatment, the amount of oxidative stress and antioxidant enzyme levels were significantly lower and higher, respectively, compared to those at the baseline levels. Corroborating these results, both endotoxin and lipid A provided protection against myocardial ischemia and reperfusion injury as evidenced by significantly improved postischemic recovery of left ventricular functions. The data presented here demonstrates that a controlled amount of oxidative stress induces the expression of intracellular antioxidants that can result in enhanced myocardial tolerance to ischemia. This suggests that myocardial adaptation to oxidative stress may be a potential tool for reduction of ischemic/reperfusion injury.

Key words

oxidative stress adaptation antioxidant reserve ischemia/reperfusion injury myocardial adaptation to ischemia endotoxin lipid A 


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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Nilanjana Maulik
    • 1
    • 2
  • Masazumi Watanabe
    • 1
    • 2
  • Daniel T. Engelman
    • 1
    • 2
  • Richard M. Engelman
    • 1
    • 2
  • Dipak K. Das
    • 1
    • 2
  1. 1.Department of SurgeryUniversity of Connecticut School of MedicineFarmingtonUSA
  2. 2.Department of SurgeryBaystate Medical CenterSpringfieldUSA

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