Expression of GTP-binding protein alpha subunits in human thymocytes
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In this report, we investigate G protein α subunit diversity in human thymocytes, utilizing common properties shared by these genes and reverse transcription-polymerase chain reaction (RT-PCR). Sequence analysis of PCR amplified gene portions, indicate the presence of members from all four G-protein families that have been described thus far. The α subunit genes identified are: Gαi1-3 and Gαz but not Gαo from the Gi family, Gαs from the Gs family, Gα11, Gαq, and Gα16 from the Gq family, and Gα12 and Gα13 from the G12 family. Also in this report we present the nucleotide and predicted amino acid sequences of the human Gα13 cloned from a thymocyte cDNA library. The sequence of the human Gα13 has not been previously reported. Comparison of this sequence with the reported murine Gα13 shows >90% identity at the deduced amino acid sequence level. We conclude that thymocytes represent a useful experimental system fo the study of G protein involvement in immune responses and lymphocyte development.
Key wordsG proteins thymocytes PCR
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