Abstract
The adenosine triphosphate (ATP) content of the pre-parasitic stages ofNippostrongylus brasiliensis, Haemonchus contortus (L1, L2 and L3) and the adults of the free-living nematode,Panagrellus redivivus, have been measured by bioluminescent photometry in aerated or nearanoxic conditions. The ATP content of the L1 and L2 stages of both parasitic species was unaltered by a lack of oxygen over a 90-min period. However, the L3 stage of both species and the adults ofP. redivivus showed a significant fall in the level of ATP within 10 min of nearanoxia. This lower level of ATP was maintained during oxygen lack but the initial content was restored on return of the nematodes to aerobic conditions. The results suggest that measurement of ATP by bioluminescent photometry offers a readily measured and sensitive indicator of the capacity of a nematode to cope with transient changes in oxygen supply without undue metabolic stress.
Similar content being viewed by others
References
Abrams BI, Mitchell MJ (1978) The role of oxygen in affecting survival and activity ofPelodera punctata (Rhabditidae) from sewage sludge. Nematologica 24:456–462
Atkinson DE, Walton GM (1967) Adenosine triphosphate conservation in biosynthetic regulation. J Biol Chem 242:3239–3241
Atkinson HJ (1980) Respiration in nematodes. In: Zuckerman BM (ed) Nematodes as biological models, vol 2. Academic Press, New York, pp 101–142
Atkinson HJ, Ballantyne AJ (1977a) Changes in the adenine nucleotide content of cysts ofGlobodera rostochiensis associated with hatching of juveniles. Ann Appl Biol 87:167–174
Atkinson HJ, Ballantyne AJ (1977b) The adenosine triphosphate content of cysts ofGlobodera pallida andG. rostochiensis as a possible quantitative estimate of field populations. Ann Appl Biol 87:407–414
Atkinson HJ, Onwuliri COE (1981)Nippostrongylus brasiliensis andHaemonchus contortus: Function of the excretory ampulla of the third stage larva. Exp Parasitol 52:191–198
Barrett J (1981) Biochemistry of parasitic helminths. Macmillan, London
von Brand T (1974) The biochemistry of parasites. Academic Press, New York
Cheah KS (1976) Electron transport system ofAscaris muscle mitochondria. In: van den Bossche H (ed) Biochemistry of parasites and host-parasite relationships. Academic Press, New York, pp 133–143
Ellenby C, Smith L (1966) Observations ofPanagrellus redivivus. J Helminthol 40:323–330
Onwuliri COE (1983) Energy metabolism in the developing larval stages ofAncylostoma tubaeforme andHaemonchus contortus: glycolytic and tricarboxylic acid cycle enzymes. Parasitology (In Press)
Roberts LW, Fairbairn D (1965) Metabolic studies on adultNippostrongylus brasiliensis (Nematoda: Trichostrongyloidea). J Parasitol 51:129–138
Sharpe MJ, Lee DL (1981) The effect of anaerobiosis on adenosine nucleotide levels inNematospiroides dubius andTrichostrongylus colubriformis in vitro. Parasitology 83:425–433
Snedecor GW, Cochran WG (1968) Statistical methods, 6th ed. Iowa University Press, USA
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Onwuliri, C.O.E. The effect of anaerobiosis on adenosine triphosphate levels in larvalNippostrongylus brasiliensis andHaemonchus contortus . Z. Parasitenkd. 70, 667–671 (1984). https://doi.org/10.1007/BF00926596
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00926596