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Alteration of collagenous protein profile in congestive heart failure secondary to myocardial infarction

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Abstract

The rat model of myocardial infarction is characterized by progressive cardiac hypertrophy and failure. Rats with infarcts greater than 30% of the left ventricle exhibited early and moderate, stages of heart failure 4 and 8 weeks after the occlusion of the left coronary artery, respectively. As heart failure is usually associated with remodeling of the extracellular matrix, a histological and biochemical study of cardiac collagenous proteins was carried out using failing hearts. Total collagen content in the right ventricle increased at 2, 4, and 8 weeks following occlusion of the left coronary artery whereas such a change in viable left ventricle was seen after 4 and 8 weeks. Total cardiac hydroxyproline concentration was increased in both right and left ventricular samples from the infarcted animals when compared to those of control; this increase was due to elevation of pepsin-insoluble collagen fraction. The myocardial noncollagenous/collagenous protein ratio was decreased in experimental right and left ventricular samples when compared to control samples. These findings suggest that an increase in cross-linking of cardiac collagen as well as disparate synthesis of collagenous and noncollagenous proteins occurs in this model of congestive heart, failure.

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Authors and Affiliations

  1. Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada

    Vaclav Pelouch, Ian M. C. Dixon, Rajat Sethi & Naranjan S. Dhalla

  2. Faculty of Medicine, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada

    Vaclav Pelouch, Ian M. C. Dixon, Rajat Sethi & Naranjan S. Dhalla

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  1. Vaclav Pelouch
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  2. Ian M. C. Dixon
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  4. Naranjan S. Dhalla
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Pelouch, V., Dixon, I.M.C., Sethi, R. et al. Alteration of collagenous protein profile in congestive heart failure secondary to myocardial infarction. Mol Cell Biochem 129, 121–131 (1993). https://doi.org/10.1007/BF00926360

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