Abstract
Mother and daughter sporocysts ofTrichobilharzia ocellata, developing in the snail hostLymnaea stagnalis, were searched for substances with antigenic similarities to the snail's haemolymph. Antisera to cell-free snail haemolymph and fractions there of were used in three different immunocytochemical staining methods, applied on sections of parasitized snails. Snail tissue was consistently stained; cercariae were stained, indicating that the applied methods were successful. Most sections through mother and daughter sporocysts were completely unstained. It is concluded that neither mother nor daughter sporocysts are masked by the antigens studied or substances mimicking these. The relevance of the present observations is discussed.
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References
Bayne CJ, Stephens JA (1983)Schistosoma mansoni andBiomphalaria glabrata share epitopes: antibodies to sporocysts bind host snail hemocytes. J Invertebr Pathol 42:221–223
Benex J, Tribouley J (1974) Mise en évidence à la surface de la cercaire deSchistosoma mansoni d'antigène possédant la caractère planorbe. CR Acad Sc Paris Série D 279: 683–685
Hall RL, Pearson JS, Wood EJ (1975) The haemocyanin ofLymnaea stagnalis. Comp Biochem Physiol B 52: 211–218
Kemp WM, Greene ND, Damian RT (1974) Sharing of Cercarienhüllen-Reaktion antigens betweenSchistosoma mansoni cercariae and adults and uninfectedBiomphalaria pfeifferi. Am J Trop Med Hyg 23: 197–202
Lie KJ (1982) Swellengrebel Lecture. Survival ofSchistosoma mansoni and other trematode larvae in the snailBiomphalaria glabrata. A discussion of the interference theory. Trop Geogr Med 34:111–122
Meuleman EA, Huyer AR, Mooij JH (1984) Maintenance of the life cycle ofTrichobilharzia ocellata via the duckAnas platyrhynchos and the pond snailLymnaea stagnalis. Neth J Zool, (in press)
Roder JC, Bourns TKR, Singhal SK (1977)Trichobilharzia ocellata: Cercariae masked by antigens of the snail,Lymnaea stagnalis. Exp Parasitol 41:206–212
Schot LPC, Boer HH (1982) Immunocytochemical demonstration of peptidergic cells in the pond snailLymnaea stagnalis with an antiserum to the molluscan cardioactive peptide FMRF-amide. Cell Tissue Res 25: 347–354
Sminia T, Boer HH (1973) Haemocyanin production in pore cells of the freshwater snailLymnaea stagnalis. Z. Zellforsch 145: 443–445
Sminia, T, De With ND, Bos JL, Van Nieuwmegen ME, Witter MP, Wondergem J (1977) Structure and function of the calcium cells of the freshwater pulmonate snailLymnaea stagnalis. Neth J Zool 27: 195–208
Sminia T, Van der Knaap WPW, Edelenbosch P (1979) The role of serum factors in phagocytosis of foreign particles by blood cells of the freshwater snailLymnaea stagnalis. Dev Comp Immunol 3:37–44
Stein PC, Basch PF (1979) Purification and binding properties of hemagglutinin fromBiomphalaria glabrata. J Invertebr Pathol 33:10–18
Stirewalt SM, Dorsey CH (1974)Schistosoma mansoni: cercarial penetration of host epidermis at the ultrastructural level. Exp Parasitol 35:1–15
Van der Knaap WPW, Boerrigter-Barendsen LH, Van der Hoeven DSP, Sminia T (1981a) Immunocytochemical demonstration of a humoral defence factor in blood cells (amoebocytes) of the pond snailLymnaea stagnalis Cell Tissue Res 219: 291–296
Van der Knaap WPW, Sminia T, Kroese FGM, Dikkeboom R (1981b) Elimination of bacteria from the circulation of the pond snailLymnaea stagnalis. Dev Comp Immunol 5: 21–32
Van der Knaap WPW, Sminia T, Schutte R, Boerrigter-Barendsen LH (1983) Cytophilic receptors for foreignness, and some factors which influence phagocytosis by invertebrate leukocytes: in vitro phagocytosis by amoebocytes of the snailLymnaea stagnalis. Immunology 48:377–383
Yoshino TP (1983) Selective acquisition of snail host antigens by larvalSchistosoma mansoni: Detection by monoclonal antibodies to snail hemocytes and hemolymph. Abstract 120, 2nd ISDCI Congress Los Angeles
Yoshino TP, Bayne CJ (1983) Mimicry of snail host antigens by miracidia and primary sporocysts ofSchistosoma mansoni. Parasite Immunol 5:317–328
Yoshino TP, Cheng TC (1978) Snail host antigens associated with the surface membranes ofSchistosoma mansoni miracidia. J Parasitol 64: 752–754
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van der Knaap, W.P.W., Boots, A.M.H., Meuleman, E.A. et al. Search for shared antigens in the schistosome-snail combinationTrichobilharzia ocellata-Lymnaea stagnalis . Z. Parasitenkd. 71, 219–226 (1985). https://doi.org/10.1007/BF00926272
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DOI: https://doi.org/10.1007/BF00926272