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Regulation of mRNA transcripts and DNA synthesis in the rat heart following intravenous injection of transforming growth factor beta1

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Abstract

Transforming Growth Factor-beta1 (TGF-β1) is expressed in the heart by muscle and non-muscle cardiac cells.In vitro, cardiac myocytes and non-muscle cells including cardiac fibroblasts and endothelial cells respond to regulatory effects of TGF-β1. Expression of TGF-β1 in the heart is subject to regulation by hemodynamic stimuli. Increased expression of mRNA transcripts for TGF-β1 has been reported in several models of cardiac hypertrophy. The objective of this study was to determine the effect of TGF-β1 in the myocardium. TGF-β1 was injected intravenously. Expression of mRNA transcripts for functional and structural proteins was determined by Northern hybridization analysis. DNA synthesis was determined by measurement of3H-thymidine incorporation into ventricular DNA. The results showed differential regulation of mRNAs for myocyte- and non-myocyte-specific proteins in the heart of TGF-β1 treated rats. Moderate but statistically significant decrease in DNA synthesis was observed in the heart of TGF-β1 treated rats (37.5%, P<0.025). Together, these data point to a physiological role for TGF-β1 in the heart. They further suggest that similar to its diversein vitro cell-specific regulatory effects, TGF-β1 may have multicellular targets in the heart. Effect of TGF-β1 alone or combined with those of other cytokines/hormones that come into play, as the result of its administration, may be responsible for altered gene expression and DNA synthesis in the myocardium. We propose that in experimental models of myocardial hypertrophy which are associated with increased expression of TGF-β1 in the heart, the contribution of regulatory effects of this growth factor to the manifestations of ventricular hypertrophy could be significant.

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Authors and Affiliations

  1. Department of Anesthesiology, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, USA

    Andreas Sigel, James A. Douglas & Mahboubeh Eghbali-Webb

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  1. Andreas Sigel
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  2. James A. Douglas
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  3. Mahboubeh Eghbali-Webb
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Sigel, A., Douglas, J.A. & Eghbali-Webb, M. Regulation of mRNA transcripts and DNA synthesis in the rat heart following intravenous injection of transforming growth factor beta1 . Mol Cell Biochem 141, 145–151 (1994). https://doi.org/10.1007/BF00926178

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