Molecular and Cellular Biochemistry

, Volume 136, Issue 2, pp 139–144 | Cite as

Hydroxyl radical-scavenging property of indomethacin

  • Kailash Prasad
  • Victor A. Laxdal


The ability of indomethacin to scavenge hydroxyl radical (.OH) using high pressure liquid chromatography (HPLC) was investigated. .OH radical was generated by photolysis of H2O2 (1.5–10 mmoles/L) with UV light and was trapped with salicyclic acid (500 nmoles). H2O2 produced .OH in a concentration-dependent manner as estimated by .OH adduct products 2,3- and 2,5-dihydroxybenzoic acid (DHBA). Indomethacin in increasing concentrations (5–600 μmoles/L) produced increasing inhibition of generation of 2,3-DHBA (7–67%) and of 2,5-DHBA (7–77%). The results indicate that indomethacin scavenges .OH in a concentration-dependent manner.

Key words

hydroxyl radical indomethacin 2,3-dihydroxybenzoic acid 2,5-dihydroxybenzoic acid photolysis of H2O2 hydroxyl radical scavenger 


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Kailash Prasad
    • 1
    • 2
    • 3
  • Victor A. Laxdal
    • 1
    • 2
    • 3
  1. 1.Department of Physiology, College of MedicineUniversity of SaskatchewanSaskatoonCanada
  2. 2.Department of Pathology, College of MedicineUniversity of SaskatchewanSaskatoonCanada
  3. 3.Royal University HospitalSaskatoonCanada

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