Molecular and Cellular Biochemistry

, Volume 136, Issue 2, pp 139–144 | Cite as

Hydroxyl radical-scavenging property of indomethacin

  • Kailash Prasad
  • Victor A. Laxdal
Article

Abstract

The ability of indomethacin to scavenge hydroxyl radical (.OH) using high pressure liquid chromatography (HPLC) was investigated. .OH radical was generated by photolysis of H2O2 (1.5–10 mmoles/L) with UV light and was trapped with salicyclic acid (500 nmoles). H2O2 produced .OH in a concentration-dependent manner as estimated by .OH adduct products 2,3- and 2,5-dihydroxybenzoic acid (DHBA). Indomethacin in increasing concentrations (5–600 μmoles/L) produced increasing inhibition of generation of 2,3-DHBA (7–67%) and of 2,5-DHBA (7–77%). The results indicate that indomethacin scavenges .OH in a concentration-dependent manner.

Key words

hydroxyl radical indomethacin 2,3-dihydroxybenzoic acid 2,5-dihydroxybenzoic acid photolysis of H2O2 hydroxyl radical scavenger 

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Kailash Prasad
    • 1
    • 2
    • 3
  • Victor A. Laxdal
    • 1
    • 2
    • 3
  1. 1.Department of Physiology, College of MedicineUniversity of SaskatchewanSaskatoonCanada
  2. 2.Department of Pathology, College of MedicineUniversity of SaskatchewanSaskatoonCanada
  3. 3.Royal University HospitalSaskatoonCanada

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