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Molecular and Cellular Biochemistry

, Volume 137, Issue 1, pp 57–60 | Cite as

Effect of aluminium (Al) on brain mitochondrial monoamine oxidase-A (MAO-A) activity — anin vitro kinetic study

  • K. S. Jagannatha Rao
  • Gazula Valeswara Rao
Article

Abstract

Effect of aluminium (Al) on rat brain mitochondrial monoamine oxidase-A (MAO-A) was studiedin vitro at three different pH (4.0, 7.4 and 9.0) values. The results have shown that Al is a non-competitive inhibitor for MAO-A. The data also showed that MAO-A inhibition by Al varies with free Al3+ concentration and different forms of Al under different pH conditions. Al altered the maximum velocity (Vmax) of MAO-A but did not affect substrate-enzyme affinity (Km). Al formed a strong chelation with the substrate (Kynuramine) (1:1).

Key words

aluminium monoamine oxidase kynuramine 

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References

  1. 1.
    Young Jr, WF Laws Jr, ER Sharbrough FW, Weinshilboum RM: Human monoamine oxidase. Arch Gen Psychiatry 43:604–609, 1986Google Scholar
  2. 2.
    Knoll J: Deprenyl — the MAO inhibitor without the “cheese effect”. Trends Neuro Sci 2:111–113, 1979Google Scholar
  3. 3.
    Benedetti MS, Dostert P: Monoamine oxidase, brain ageing and degenerative diseases. Biochem Pharmacol 38:555–561, 1989Google Scholar
  4. 4.
    Tsuzuki Y, Marquis JK: Investigations of the interaction of aluminium with bovine plasma monoamine oxidase. Bull Environ Contam Toxicol 34:451–458, 1985Google Scholar
  5. 5.
    Boegman RJ, Bates LA: Neurotoxicity of aluminium. Can J Physiol 62:1010–1014, 1984Google Scholar
  6. 6.
    Singh MK, Srivastava MN: Stepwise formation of beryllium (II) and aluminium (III) chelates with aspartic and glutamic acids. J Inorg Nucl Chem 34:567–573, 1972Google Scholar
  7. 7.
    Deloncle R, Guillard O: Mechanism of Alzheimer's disease arguments for a neurotransmitter complex implications. Neurochem Res 15: 1239–1245, 1990Google Scholar
  8. 8.
    Wisniewski HM, Sturman JA: In: H.J. Gitelman (ed.), Marcel Dekker, Inc. New York, 1989, pp 125–165Google Scholar
  9. 9.
    Martin BR: The chemistry of aluminium as related to biology and medicine. Clin Chem 32:1797–1806, 1986Google Scholar
  10. 10.
    Clark JB, Nicklas WJ: The metabolism of rat brain mitchondria. J Biol Chem 245:4724–4731, 1970Google Scholar
  11. 11.
    Knoll J, Magyar K: In: E. Costa & M. Sandler (eds), Advances in biochemical psychopharmacology, Vol. 5, Raven Press, New York, 1972, pp 393–408Google Scholar
  12. 12.
    Krajl M: A rapid microfluorimetric determination of monoamine oxidase. Biochem Pharmacol 14:1683–1685, 1965Google Scholar
  13. 13.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265–275, 1951Google Scholar
  14. 14.
    Jagannatha Rao KS: In: A.M. Goldberg (ed.),In vitro toxicology —Approaches for validation. Mary Ann Liebert, New York, 1987, pp 421–451Google Scholar
  15. 15.
    Fowler CJ, Callingham BA, Mantle TJ, Tipton KF: Monoamine oxidase A and B: A useful concept. Biochem. Pharmacol 27:97–101, 1978Google Scholar
  16. 16.
    Lineweaver H, Burk D: Determination of enzyme dissociation constants. J Am Chem Soc 56:658–666, 1934Google Scholar
  17. 17.
    Williams MW, Hoeschele JD, Turner JE, Jacobson KB, Christie NT, Paton CL, Smith LH, Witschi HR, Lee EH: Chemical softness and acute metal toxicity in mice and drosophila. Toxicol Appl Pharmacol 63:461–469, 1982Google Scholar
  18. 18.
    Rajan KS, Davis JM, Colburn RW, Jarke FH: Metal chelates in the storage and transport of neurotransmitters interaction of Cu with ATP and biogenic amines. J Neuro Chem 19:1099–1116, 1972Google Scholar
  19. 19.
    Smith RW: In: ACS Advances in chemistry series, Chap. 10, Vol. 106, American Chemical Society, Washington DC, 1971, pp 250–279Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • K. S. Jagannatha Rao
    • 1
  • Gazula Valeswara Rao
    • 1
  1. 1.Department of Biochemistry and NutritionCentral Food Technological Research InstituteMysoreIndia

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