Abstract
In an attempt to improve the specificity of the crudeTaenia saginata antigen for the immunodiagnosis of bovine cysticercosis, a major and highly immunogenic fraction (F10), responsible for the formation of the typical “long band” reaction in immunoelectrophoresis, has been isolated fromT. saginata proglottides by immunoaffinity chromatography. The immunoadsorbant was prepared by coupling a specifically raised hyperimmune serum (HIS) anti-F10 to Sepharose 4B.
The purity of the isolated F10 was demonstrated by immunoprecipitation reactions. The HIS anti-F10, however, cross-reacted with several larval and adultTaenia spp. Consequently, F10 showed cross-reactions with the sera of animals infected with hydatid cysts or larvalT. hydatigena. F10 also reacted with HIS anti-F5 (Echinococcus granulosus) but was shown to be non-identical with the well known F5 ofE. granulosus. These data prove that F10 ofT. saginata was not species-specific but showed a group specificity for the taeniid family — a situation analogous to F5 ofE. granulosus.
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Geerts, S., Vervoort, T., Kumar, V. et al. Isolation of fraction No. 10 fromTaenia saginata and evaluation of its specificity for the diagnosis of bovine cysticercosis. Z. Parasitenkd. 66, 201–206 (1981). https://doi.org/10.1007/BF00925727
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DOI: https://doi.org/10.1007/BF00925727