Abstract
Nuclear division and microgametogony ofEimeria nieschulzi were studied by transmission electron microscopy. All nuclear divisions occurred in close proximity to the gamont surface with four centrioles situated between the nuclear envelope and the gamont plasmalemma. During early nuclear divisions, each nucleus had a rod or hourglass-shaped nucleolus, indicating that the nucleolus probably pinched in two during nuclear division. Nuclear divisions occurred by nearly centrally located intranuclear spindles. Two centrioles were associated with each centrocone. Spindle microtubules, which originated from each centrocone, either traversed to the other centrocone or terminated at kinetochores. Four to 6 chromosome-like structures were present in each dividing nucleus. The formation of 50 to 100 biflagellate microgametes occurred at the margin of the microgamont by a process similar to that described for other coccidian species. Microgametes were limited by a single membrane with a prominent glycocalyx, 13 nm thick.
Similar content being viewed by others
References
Becker B, Mehlhorn H, Heydorn AO (1979) Light and electron microscopic studies on gamogony and sporogony of 5Sarcocystis species in vivo and in tissue cultures. Zbl Bakt Hyg I Abt Orig A 244:394–404
Canning EU, Anwar M (1968) Studies on meiotic division in coccidial and malarial parasites. J Protozool 15:290–298
Danforth HD, Hammond DM (1972) Stages of merogony in multinucleate merozoites ofEimeria magna Pérard, 1925. J Protozool 19:454–457
Dobell C (1925) The life history and chromosome cycle ofAggregata eberthi (Protozoa:Sporozoa: Coccidia). Parasitology 17:1–136
Dubremetz JF (1973) Etude ultrastructurale de la mitose schizogonique chez la coccidiaEimeria necatrix (Johnson 1930). J Ultrastruct Res 42:354–376
Dubremetz JF, Elsner YY (1979) Ultrastructure study of schizogony ofEimeria bovis in cell cultures. J Protozool 26:367–376
Ferguson DJP, Hutchison WM, Dunachie JF, Siim J Chr (1974) Ultrastructural study of early stages of asexual multiplication and microgametogony ofToxoplasma gondii in the small intestine of the cat. Acta pathol microbiol scand Sect B 82:167–181
Fernando MA (1973) Fine structural changes associated with microgametogenesis ofEimeria acervulina in chickens. Z Parasitenkd 43:33–42
Grell KG (1953). Entwicklung und Geschlechtsbestimmung vonEucoccidium dinophili. Arch Protistenkd 99:156–186
Hammond DM, Roberts WL, Youssef NN, Danforth HD, (1973) Fine structure of the intranuclear spindle poles inEimeria callospermophili andE. magna. J Parasitol 59:581–584
Kelley GL, Hammond DM (1972) Fine structural aspects of early development ofEimeria ninakohlyakimovae in cultured cells. Z Parasitenkd 38:271–284
Kelley GL, Hammond DM (1973) Fine structural aspects of nuclear division and merogony ofEimeria ninakohlyakimovae in cultured cells. J Parasitol 59:1071–1080
Lepage G (1940) The study of coccidiosis (Eimeria caviae (Sheather 1924)) in the guinea pig. Part II. The live history. Vet J 96:242–254; 280–295
Mehlhorn H, Heydorn AO (1979) Electron microscopical study on gamogony ofSarcocystis suihominis in human tissue cultures. Z Parasitenkd 58:97–113
Scholtyseck E (1963) Untersuchungen über die Kernverhältnisse und das Wachstum bei Coccidiomorphen unter besonderer Berücksichtigung vonEimeria maxima. Z Parasitenkd 22:428–474
Scholtyseck E (1973) Ultrastructure In: Hammond DM (ed) The Coccidia Univ Park Press, Baltimore pp 81–144
Scholtyseck E, Mehlhorn H, Hammond DM (1972) Electron microscope studies of microgametogenesis in coccidia and related groups. Z Parasitenkd 38:95–131
Scholtyseck E, Chobotar B, Senaud J, Ernst JV (1977) Fine structure of microgametogenesis ofEimeria ferris Levine and Ivens 1965 inMus musculus. Z Parasitenkd 51:229–240
Speer CA, Danforth HD (1976) Fine structural aspects of microgametogenesis ofEimeria magna in rabbits and in kidney cell cultures. J Protozool 23:109–115
Speer CA, Hammond DM (1970) Nuclear divisions and refractile-body changes in sporozoites and schizonts ofEimeria callospermophili in cultured cells. J Parasitol 56:461–467
Vetterling JM, Pacheco ND, Fayer R (1973) Fine structure of gametogony and oocyst formation inSarcocystis sp. in cell culture. J Protozool 20:613–621
Zaman V, Colley FC (1975) Light and electron microscopic observations of the life cycle ofSarcocystis orientalis sp.n. in the rat (Rattus norvegicus) and the malayian reticulated python (Phython reticulatus). Z Parasitenkd 47:169–185
Author information
Authors and Affiliations
Additional information
Based on a thesis submitted by G.J. Sibert to the Graduate School of the University of Montana in partial fulfillment of the requirements for the Master of Science degree
Rights and permissions
About this article
Cite this article
Sibert, G.J., Speer, C.A. Fine structure of nuclear division and microgametogony ofEimeria nieschulzi Dieben, 1924. Z. Parasitenkd. 66, 179–189 (1981). https://doi.org/10.1007/BF00925725
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00925725