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PCR-based analysis of voltage-gated K+ channels in vascular smooth muscle

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Abstract

Irregularities in K+ currents form the basis of several cardiovascular dysfunctions, among which are arrhythmias and vasospasms. The developmental regulation of voltage-gated K+ channels, however, has been difficult to study. A novel approach was therefore employed to examine these channels in muscle tissue. Primers for a PCR-based analysis were designed using published nucleic acid sequences for voltage-gated K+ channels. Final selection of the primer pairs was based on the homology present in the S4 and H5 transmembrane domains. A specific band was amplified with these primers using RNA isolated from both rat A10 vascular smooth muscle cells and rat heart tissue.

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Author notes

  1. Ginette Lamontagne

    Present address: CHUL Research Centre, 2705 Boulevard Laurier, Ste-Foy, Quebec

  2. Normand Leblanc

    Present address: Research Centre, Montreal Heart Institute, 5000 east, Belanger Street, Montreal, Quebec, Canada

Authors and Affiliations

  1. Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, 351 Tache Avanue, R2H 2A6, Winnipeg, MB, Canada

    Peter Zahradka, Karen D. Harris, Ginette Lamontagne & Normand Leblanc

  2. Department of Physiology, University of Manitoba, Canada

    Peter Zahradka, Karen D. Harris & Normand Leblanc

  3. Department of Biochemistry and Molecular Biology, University of Manitoba, Canada

    Barbara Triggs-Raine

Authors
  1. Peter Zahradka
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  2. Karen D. Harris
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  3. Barbara Triggs-Raine
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  4. Ginette Lamontagne
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  5. Normand Leblanc
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Zahradka, P., Harris, K.D., Triggs-Raine, B. et al. PCR-based analysis of voltage-gated K+ channels in vascular smooth muscle. Mol Cell Biochem 145, 39–44 (1995). https://doi.org/10.1007/BF00925711

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  • DOI: https://doi.org/10.1007/BF00925711

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