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Regulatory effect of various steroid hormones on the incorporation and metabolism of [14C]stearate in rat hepatoma cells in culture

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Abstract

We have examined the incorporation and metabolism of [14C] stearic acid within the total lipids of HTC rat-hepatoma cells in suspension culture in presence and in absence of steroidal hormone stimulation. Both, glucocorticoids (dexamethasone, cortisol and corticosterone) and mineralocorticoids (deoxycorticosterone and aldosterone) as well as the estrogen β-estradiol and the androgen testosterone enhanced the extent of Δ9 desaturation to oleic acid of the saturated precursors, whereas only the two mineralocorticoids affected the incorporation rate of the exogenous acid into total cellular lipids, thus promoting a little stimulation. Furthermore, all the hormones tested increased the radiolabelling of the total cellular phospholipids except deoxycorticosterone and testosterone, the former having no effect and the latter exerting a moderate inhibition. On the other hand, the incorporation of14C into neutral lipids was stimulated by testosterone, in contrast to the inhibition of this parameter observed exclusively with either the mineralocorticoids or the estrogen. Within the phospholipid subclasses, the radiolabelling of phosphatidylcholine was augmented by means of all the steroids tested save deoxycorticosterone and testosterone, whereas phosphatidylethanolamine exhibited a decrease only in the presence of testosterone. In a similar fashion, within the neutral lipids, the predominating triglyceride fraction was preferentially labelled—at the expense of other subclasses of lesser abundance—upon treatment with the steroids except aldosterone, which exerted no effect. The results obtained were correlated with those changes observed in the mass distribution of the different lipid subclasses either with or without prior hormonal stimulation.

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Abbreviations

ALD:

aldosterone

ANOVAR:

analyses of variance

CE:

cholesterol esters

Cl:

cortisol

CLN:

cardiolipin

Cst:

corticosterone

CH:

cholesterol

DG:

diacylglycerides

DOC:

11-deoxycorticosterone

Dx:

dexamethasone phosphate salt

ßE:

17-β-estradiol

FAME:

fatty acid methyl esters

GLC:

gas-lipid chromatography

HEPES:

N-2-hydroxyethyl-piperazine-N-2-ethanosulfonic acid

HTC:

hepatoma tissue culture

IMEM-Zo:

improved minimal essential medium-Zinc option

LPC:

lysophosphatidyl-choline

LPL:

lysophospholipids

MG:

monoacylglycerides

NEFA:

free fatty acids

PC:

phosphatidyl-choline

PE:

phosphatidyl-ethanolamine

PG:

phosphatidyl-glycerol

PI:

phosphadyl-inositol

PS:

phosphadidyl-serine

SM:

sphingomyelin

TG:

triacylglycerides

TLC:

thin-layer chromatography

Tst:

testosterone

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Authors and Affiliations

  1. Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CONICET-UNLP, Facultad de Ciencias Médicas, 60 y 120, 1900, La Plata, Argentina

    Carlos Alberto Marra & María J. T. de Alaniz

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  1. Carlos Alberto Marra
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  2. María J. T. de Alaniz
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Members of the Carrera del Investigador Cientifico, CONICET, Argentina.

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Marra, C.A., de Alaniz, M.J.T. Regulatory effect of various steroid hormones on the incorporation and metabolism of [14C]stearate in rat hepatoma cells in culture. Mol Cell Biochem 145, 1–9 (1995). https://doi.org/10.1007/BF00925706

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