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Ultrastructural localization of the circulating cathodic antigen in the digestive tract of various life-cycle stages ofSchistosoma mansoni

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Abstract

In the present study the ultrastructural localization of the schistosome-derived circulating cathodic antigen (CCA) within the digestive tract of various life-cycle stages of the blood flukeSchistosoma mansoni has been determined. Use was made of CCA-specific monoclonal antibodies in a two-step gold-labeling procedure. The following results were obtained: In cercariae gold label was found in the cytoplasm and in the luminal surface coat of the gut epithelium. The oesophagus of this life-cycle stage of the parasite showed minimal CCA reactivity, as gold label was limited to the luminal surface coat, locating proximally to the gut. In 3 1/2-week-old worms and in adult male and female worms CCA was demonstrable in the Golgi apparatus, in cytoplasmic vesicles and in the luminal surface coat; in the caudal quarter of the gut of adult worms CCA was also present in lysosome-like bodies. The oesophagus of these worm preparations did not show any CCA reactivity. In miracidia CCA was not present.

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Authors and Affiliations

  1. Laboratory for Parasitology, University of Leiden, Wassenaarseweg 62, NL-2333 AL, Leiden, The Netherlands

    R. de Water & A. M. Deelder

  2. Laboratory for Electron Microscopy, University of Leiden, Rijnsburgerweg 10, NL-2333 AA, Leiden, The Netherlands

    J. A. M. Fransen

Authors
  1. R. de Water
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  2. J. A. M. Fransen
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  3. A. M. Deelder
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Additional information

This investigation received financial support from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases

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de Water, R., Fransen, J.A.M. & Deelder, A.M. Ultrastructural localization of the circulating cathodic antigen in the digestive tract of various life-cycle stages ofSchistosoma mansoni . Z. Parasitenkd. 72, 635–646 (1986). https://doi.org/10.1007/BF00925485

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  • DOI: https://doi.org/10.1007/BF00925485

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