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Assessment of the in vitro growth ofPlasmodium falciparum using fluorometry

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Abstract

The growth ofPlasmodium falciparum in vitro was quantitatively assessed by applying fluorometry using ethidium bromide. The fluorescence intensity of parasites stained with this dye was found to parallel the uptake of3H-hypoxanthine into nucleic acids during one growth cycle of development. The assay system can be used as a substitute of morphological and radiometric methods in drug-sensitivity tests and for the screening of antimalarials.

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Authors and Affiliations

  1. Department of Parasitology, Gunma University School of Medicine, 3-39-22 Showa-machi, 371, Maebashi, Japan

    Jun'ichi Tamura & Seiji Waki

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  1. Jun'ichi Tamura
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  2. Seiji Waki
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Tamura, J., Waki, S. Assessment of the in vitro growth ofPlasmodium falciparum using fluorometry. Z. Parasitenkd. 72, 595–597 (1986). https://doi.org/10.1007/BF00925480

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  • DOI: https://doi.org/10.1007/BF00925480

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