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An inexpensive easily available replacement for foetal calf serum in media for the in vitro cultivation ofLeishmania spp.

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Abstract

A replacement for foetal calf serum (FCS), made from heat-treated adult serum, in culture media designed for the in vitro cultivation of leishmanial promastigotes is described. The growth of 12 World Health Organization recommended strains ofLeishmania in media containing heat-treated adult serum was compared with that obtained in media containing FCS. Growth of the promastigotes in the media with the FCS replacement was as good as or slightly better than that with FCS.

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Authors and Affiliations

  1. Department of Medical Protozoology, London School of Hygiene and Tropical Medicine, WHO International Collaborating Centre for the Cryopreservation of Parasitic Protozoa and International Reference Centre for Leishmania, Keppel Street, W1E 7HT, London, UK

    David A. Evans

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  1. David A. Evans
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Evans, D.A. An inexpensive easily available replacement for foetal calf serum in media for the in vitro cultivation ofLeishmania spp.. Z. Parasitenkd. 72, 567–572 (1986). https://doi.org/10.1007/BF00925475

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  • DOI: https://doi.org/10.1007/BF00925475

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