The use of percoll gradients, elutriator rotor elution, and mithramycin staining for the isolation and identification of intraerythrocytic stages ofPlasmodium berghei
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Intraerythrocytic parasites ofPlasmodium vinckei andPlasmodium berghei were separated according to their developmental stages using discontinuous Percoll gradients. Contaminating nucleated blood cells such as leukocytes were removed by elutriation centrifugation. The stages were unequivocally identified in smears using a newly developed DNA-specific staining procedure with mithrmycin and fluorescence microscopy. This stain can also be used to detect parasites in human blood of very low parasitemias. The combination of methods described has many possible applications in immunologic and biochemical parasite research.
KeywordsMalaria Infected Erythrocyte Percoll Gradient Gradient Fraction Plasmodium Berghei
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- Eling W (1977) Ficoll fractionation for the separation of parasitized erythrocytes from malaria infected blood. Bull WHO 57: 105–114Google Scholar
- Heidrich HG (1970) Isolation of pure inner and outer mitochondrial membranes in preparative scale. In: Advances in automated chemistry: proceedings of the international Technicon congress 1969, MEDIAD-Technicon, Tarrytown-New York pp 347–350Google Scholar
- Heidrich HG, Rieckmann KH (1980) Free-flow electrophoresis separation of intracellular parasites (Plasmodium falciparum) from culture. Eur J Cell Biol 22: 516Google Scholar
- Pertoft H, Laurent TC (1979) Isopycnic separation of cells and cell organelles by centrifugation in modified colloidal silica gradients. In: Catsimpolas N (ed) Methods of cell separation. Plenum, New York, pp 25–56Google Scholar
- Sodeman TM (1970) The use of fluorochromes for the detection of malaria parasites. Am J Trop Med Hyp 19: 40–45Google Scholar