TCA manual / Tissue Culture Association

, Volume 5, Issue 4, pp 1161–1163 | Cite as

Production, growth and handling of multicellular tumor spheroids

  • Peter W. Woodman
  • Michael D. Armour

Key words

multicellular tumor spheroids agar-base layers 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Sutherland, R. M., and R. E. Durand. 1976. Radiation response of multicell spheroids —anin vitro tumor model. Curr. Top. Radiat. Res. 11: 87–139.Google Scholar
  2. 2.
    Durand, R. E., and R. M. Sutherland. 1972. Effects of intercellular contact on radiation damage. Exp. Cell Res.71: 75–80.Google Scholar
  3. 3.
    Folkman, J., and M. Hochberg. 1973. Selfregulation of growth in three dimensions. J. Exp. Med. 138: 745–753.Google Scholar
  4. 4.
    Sutherland, R. M., J. A. McCredie, and W. R. Inch. 1971. Growth of multicell spheroids in tissue culture as a model for nodular carcinoma. J. Nat. Cancer Inst. 46: 113–120.Google Scholar
  5. 5.
    Sutherland, R. M., and R. E. Durand. 1973. Hypoxic cells in anin vitro tumor model. Int. J. Radiat. Biol. 23: 235–246.Google Scholar
  6. 6.
    Yuhas, J. M., A. P. Li, A. O. Martinez, and A. J. Ladman. 1977. A simplified method for the production and growth of multicellular tumor spheroids. Cancer Res. 37: 3639–3643.Google Scholar
  7. 7.
    Yuhas, J. M., A. E. Tarleton, and K. B. Molzen. 1978. Multicellular tumor spheroid formation by breast cancer cells isolated from different sites. Cancer Res. 38: 2486–2491.Google Scholar
  8. 8.
    Putman, D. L., D. K. Park, J. S. Rhim, A. F. Steur, and R. C. Ting. 1977. Correlation of cellular aggregation of transformed cells with their growth in soft agar and tumorigenic potential. Proc. Soc. Exp. Biol. Med. 155: 487–494.Google Scholar
  9. 9.
    Handschack, W., J. Kopp, and C. Pita. 1977. A simple method for cloning and replica plating of mammalian cells using multicellular spheroids. Acta Biol. Med. Ger. 36: 1429–1434.Google Scholar
  10. 10.
    Woodman, P. W. 1979. Multicellular tumor spheroids (MTS) asin vitro-models for human colorectal carcinomas (abstr.). Proc. Am. Assoc. Cancer Res. 20: 40.Google Scholar

Copyright information

© Kluwer Academic Publishers 1979

Authors and Affiliations

  • Peter W. Woodman
    • 1
  • Michael D. Armour
    • 1
  1. 1.Division of Biochemical and Clinical PharmacologySt. Jude Children's Research HospitalMemphis

Personalised recommendations