Osmotic state of lenses in three dominant murine cataract mutants

  • Jochen Graw
  • Peter Reitmeir
  • Andrea Wulff
Laboratory Investigations


Three newly detected dominant cataract mutations (Asc-1, Cat-3vao,Tcm) were investigated for effects on osmotic alterations in the lenses of heterozygotes. The lens wet weight was reduced in two mutant lines (Cat-3vao andTcm), and the water content in the lenses of theCat-3vao mice was increased. Moreover, in the cataractous lenses fromCat-3vao mice, the sodium-potassium-adenosine triphosphatase (Na+ -K+-ATPase) activity was enhanced and the ATP concentration, correspondingly decreased. The osmotic variations observed in theCat-3vao mutants might have been due to a metabolic response to the yet unknown, primary pathological event. The lenses of the other two mutant lines (Asc-1 andTcm) revealed no alterations that could be related to osmotic stress. In no mutant line investigated could a decrease in Na+-K+-ATPase activity be demonstrated that was similar to the causative factor in the Nakano mutant line. TheCat-3vao mice exhibited some similarities to the Philly mutant line.


Public Health Cataract ATPase Activity Osmotic Stress Causative Factor 
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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Jochen Graw
    • 1
  • Peter Reitmeir
    • 2
  • Andrea Wulff
    • 2
  1. 1.GSF-Institut für SäugetiergenetikNeuherbergGermany
  2. 2.GSF-Institut für Medizinische Informatik und SystemforschungNeuherbergGermany

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