TCA manual / Tissue Culture Association

, Volume 5, Issue 2, pp 1081–1085 | Cite as

A spectrophotometric assay for hydrogen peroxide in tissue culture medium

  • William G. Taylor
  • Richard F. Camalier
  • Marilyn J. Taylor

Key words

hydrogen peroxide light chromosome breaks cultured cells culture medium 


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  1. 1.
    Wang, R. J. 1976. Effect of room fluorescent light on the deterioration of tissue culture medium. In Vitro 12: 19–22.Google Scholar
  2. 2.
    Wang, R. J., and B. T. Nixon. 1978. Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissueculture medium irradiated with “daylight” fluorescent light. In Vitro 14: 715–722.Google Scholar
  3. 3.
    Parshad, R., K. K. Sanford, W. G. Taylor, R. E. Tarone, G. M. Jones, and A. E. Baeck. 1979. Effect of intensity and wavelength of fluorescent light on chromosome damage in cultured mouse cells. Photochem. Photobiol. 29: 971–975.Google Scholar
  4. 4.
    Peterkofsky, B., and W. Prather. 1977. Cytotoxicity of ascorbate and other reducing agents towards cultured fibroblasts as a result of hydrogen peroxide formation. J. Cell. Physiol. 90: 61–70.Google Scholar
  5. 5.
    Andrae, U., and H. Greim. 1979. Induction of DNA repair replication by hydroxyurea in human lymphoblastoid cells mediated by liver microsomes and NADPH. Biochem. Biophys. Res. Commun. 87: 50–58.Google Scholar
  6. 6.
    Parshad, R., K. K. Sanford, G. M. Jones, and R. E. Tarone. 1978. Fluorescent light-induced chromosome damage and its prevention in mouse cells in culture. Proc. Nat. Acad. Sci. U.S.A. 75: 1830–1833.Google Scholar
  7. 7.
    Gantt, R., R. Parshad, R. A. G. Ewig, K. K. Sanford, G. M. Jones, R. E. Tarone, and K. W. Kohn. 1978. Fluorescent light-induced DNA crosslinkage and chromatid breaks in mouse cells in culture. Proc. Nat. Acad. Sci. U.S.A. 75: 3809–3812.Google Scholar
  8. 8.
    Parshad, R., K. K. Sanford, R. E. Tarone, G. M. Jones, and A. E. Baeck. 1979. Increased susceptibility of mouse cells to fluorescent light-induced chromosome damage after long term culture and malignant transformation. Cancer Res. 39: 929–933.Google Scholar
  9. 9.
    Gantt, R., G. Jones, E. V. Stevens, A. E. Baeck, and K.K. Sanford. Visible light-induced DNA crosslinks in cultured mouse and human cells. Biochim. Biophys. Acta, in press.Google Scholar
  10. 10.
    Patrick, W. A., and H. B. Wagner. 1949. Determination of hydrogen peroxide in small concentrations. A spectrophotometric method. Anal. Chem. 21: 1279–1280.Google Scholar
  11. 11.
    Quagliano, J. V., and L. M. Vallarino. 1969.Chemistry. Prentice-Hall, Inc., Englewood Cliffs, N.J., pp. 208–211.Google Scholar
  12. 12.
    Johnson, R. M., and I. W. Siddigi. 1970.The Determination of Organic Peroxides. Pergamon Press, New York, pp. 50–52.Google Scholar
  13. 13.
    Schumb, W. C., C. N. Satterfield, and R. L. Wentworth. 1955,Hydrogen Peroxide. Reinhold Publishing Co., New York, pp. 398–399.Google Scholar
  14. 14.
    Sully, B. D., and P. L. Williams. 1962. The analysis of solutions of per-acids and hydrogen peroxide. Analyst (London) 87: 653–657.Google Scholar

Copyright information

© Tissue Culture Association 1979

Authors and Affiliations

  • William G. Taylor
    • 1
  • Richard F. Camalier
    • 1
  • Marilyn J. Taylor
    • 2
  1. 1.In Vitro Carcinogenesis Section Laboratory of Cellular and Molecular Biology National Cancer InstituteNational Institutes of HealthBethesda
  2. 2.College of MedicineHoward UniversityWashington, D.C.

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