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Inflammation

, Volume 12, Issue 5, pp 503–514 | Cite as

Production of lipoxygenase metabolites of eicosapentaenoic acid by bovine alveolar macrophages in vitro

  • W. W. Laegreid
  • S. M. Taylor
  • R. M. Silflow
  • K. M. Straub
  • R. G. Breeze
  • H. D. Liggitt
  • R. W. Leid
Original Articles

Abstract

Lipoxygenase metabolites of arachidonic acid (AA), the leukotrienes (LTs), and hydroxyeicosatetraenoic acids (HETEs) are potent proinflammatory mediators. Release of LTs and HETEs by bovine alveolar macrophages (BAMs) was measured by reverse-phase high performance liquid chromatography. LTB4 (1.1±0.2 ng/106 cells) and 5-HETE (2.2±0.2 ng/106 cells) were the major metabolites calcium ionophore A23187-stimulated BAMs produced from endogenous AA. The tritiated forms of these compounds and their precursor fatty acids were produced following incorporation of [3H]AA into the cells and stimulation by calcium ionophore A23187. Incorporation of an alternative substrate, [3H]eicosapentaenoic acid ([3H]EPA) into BAMs incubated in parallel resulted in production of [3H]LTB5 and [3H]5-hydroxyeicosapentaenoic acid (5-HEPE). Equivalent amounts of [3H]AA and [3H]EPA and of [3H]LTB4 and homologous [3H]LTB5 were released. BAM produced significantly greater amounts of [3H]5-HEPE than [3H]5-HETE, however. These findings indicate that the BAM 5-lipoxygenase is capable of metabolizing EPA to LTB5 and 5-HEPE, with the production of 5-HEPE preferred over 5-HETE.

Keywords

Liquid Chromatography High Performance Liquid Chromatography Arachidonic Acid A23187 Equivalent Amount 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • W. W. Laegreid
    • 1
  • S. M. Taylor
    • 1
  • R. M. Silflow
    • 1
  • K. M. Straub
    • 2
  • R. G. Breeze
    • 1
  • H. D. Liggitt
    • 1
  • R. W. Leid
    • 1
  1. 1.Laboratory of Molecular Inflammation Department of Veterinary Microbiology and PathologyWashington State UniversityPullman
  2. 2.Department of Drug MetabolismSmith Kline and French LaboratoriesSwedeland

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