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The effect of EGb 761 on retinal lipid peroxidation and glutathione peroxidase level in experimental lens induced uveitis

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Abstract

An acute lens-induced necrotizing intraocular inflammation was produced in pigmented guinea pigs. Treatment of these animals by 100 mg/kg/day EGb 761 a free oxygen radical scavenger for 10 days, reduced retinal lipid peroxidation (p>0.05) and increased the retinal glutathione peroxidase level (p > 0.05). Although not significantly, these findings suggest that EGb 761 could be combined with other antiinflammatory drugs and may be beneficial in the treatment of uveitis.

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References

  1. Mittag T. Role of oxygen radicals in ocular inflammation and cellular damage. Exp Eye Res 1984; 39: 759–69.

    Google Scholar 

  2. Weiss SJ, Ward PA. Immune complex induced generation of oxygen metabolites by human neutrophils. J Immunol 1982; 129: 209–13.

    Google Scholar 

  3. Marak GE, Rao NA, Scott JM, Duque R, Ward PA. Free radicals and phacoanaphylaxis. In: O'Connor GR, Chardler J, editors. Advances in immunology and immunopathology of the eye. New York: Masson, 1985: 144–5.

    Google Scholar 

  4. Babior BM. Oxygen dependent microbial killing by phagocytes. New Eng J Med 1978; 298: 659–68.

    Google Scholar 

  5. Atalla LR, Sevanian A, Rao NA. Immunohistochemical localization in ocular tissue, an electron microscopic cytochemical study. Curr Eye Res 1988; 7: 931–6.

    Google Scholar 

  6. Sery TW, Vogel AW, Folberg R, Petrillo R. Oxygen free radicals in ocular inflammatory disease. In: Saari KM, editor. Uveitis update. Amsterdam: Elsevier Science Publishers, 1984: 39–45.

    Google Scholar 

  7. Marak GE, Kozak Y, Faure JP. Free radicals and antioxidants in the pathogenesis of eye diseases. In: Emerit I, editor. Antioxidants in therapy and preventive medicine. New York: Plenum Press, 1990: 513–27.

    Google Scholar 

  8. Bhuyan KC, Bhuyan DK. Molecular mechanism of cataractogenesis: Toxic metabolites of oxygen as initiators of lipid peroxidation and cataract. Curr Eye Res 1984;3: 67–81.

    Google Scholar 

  9. Stone WL, Dratz EA. Selenium and non-selenium glutathione peroxidase activities in selected ocular and non-ocular rat tissues. Exp Eye Res 1982; 35: 405–12.

    Google Scholar 

  10. Anderson RE, Andrews LM. Biochemistry of retinal photoreceptor membranes in vertebrates. In: Westfall J, editor. Visual cells in evolution. New York: Raven Press, 1981: 1–22.

    Google Scholar 

  11. Fliesler SJ, Anderson RE. Chemistry and metabolism of lipids in the vertebrate retina. Prog Lipid Res 1983; 22: 79–131.

    Google Scholar 

  12. Anderson RE, Maude MB, Nielsen JC. Effect of lipid peroxidation on rhodopsin regeneration. Curr Eye Res 1985; 4: 56–71.

    Google Scholar 

  13. Tappel A. Lipid peroxidation and fluorescent molecular damage to membranes. In: Trump B, Arstila A, editors. Pathobiology of cell membranes. New York: Academic Press, 1975: 145–70.

    Google Scholar 

  14. Lowry OH. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193: 265–75.

    Google Scholar 

  15. Beuge JA. Microsomal lipid peroxidation. Methods Enzymology 1978; 52: 302–10.

    Google Scholar 

  16. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 158–69.

    Google Scholar 

  17. Rao NA, Calandra AJ, Sevanian A, Boure B, Delmage JM, Marak GE. Modulation of lens-induced uveitis by superoxide dismutase. Ophthal Res 1986; 18: 41–6.

    Google Scholar 

  18. Rehan A, Johnson KJ, Wiggins RC, Kunkel RG, Ward PA. Evidence for the role of oxygen radicals in acute nephrtoxic nephritis. Lab Invest 1984; 51: 396–403.

    Google Scholar 

  19. Bhuyan KC, Bhuyan DK. Regulation of hydrogen peroxide in eye tumors; effect of 3-amino-IH-1,2,4-triazole on catalase and glutathione peroxidase of rabbit eye. Biochem Biophys Acta 1977; 497: 641–51.

    Google Scholar 

  20. Armstrong D, Santangels G, Comle E. The distribution of peroxide regulating enzymes in the canine eye. Curr Eye Res 1981; 1: 225–42.

    Google Scholar 

  21. Flohe C. Glutathione peroxidase into focus. In: Pryor WA, editor. Free radicals in biology. New York: Academic Press, 1982: 254–61.

    Google Scholar 

  22. Rao NA, Romero JL, Sevanian A, Fernandez MA, Wong C, Ward PA, Marak GE. Anti-inflammatory effect of glutathione peroxidase on experimental lens-induced uveitis. Ophthalmic Res 1988; 20: 213–9.

    Google Scholar 

  23. Gritz DC, Montes C, Atalla LR, Wu GS, Sevanian A, Rao NA. Histochemical localization of superoxide production in experimental autoimmune uveitis. Curr Eye Res 1991; 10: 927–31.

    Google Scholar 

  24. Rao NA, Fernandez MA, Cid LL, Romero JL, Sevanian A. Retinal lipid peroxidation in experimental uveitis. Arch Ophthalmol 1987; 105: 1712–6.

    Google Scholar 

  25. Pincemail J, Thirion A, Dupuis M, Braquet P, Drieu K, Deby C. Ginkgo biloba extract inhibits oxygen species production generated by phorbol myristate acetate stimulated human leukocytes. Experientia 1987; 43: 181–4.

    Google Scholar 

  26. Pincemail J, Dupuis M, Nasr C, Hans P, Haag-Berrurier M, Anton R, Deby C. Superoxide anion scavenging effect and Superoxide dismutase activity of ginkgo biloba extract. Experientia 1989; 45: 708–12.

    Google Scholar 

  27. Szabo ME, Droy-Lefaix MT, Doly M, Carre C, Braquet P. Ischemia and reperfusion-induced histologic changes in the rat retina. Invest Ophthalmol Vis Sci 1991; 32: 1471–8.

    Google Scholar 

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Bilgihan, A., Aricioğlu, A., Bilgihan, K. et al. The effect of EGb 761 on retinal lipid peroxidation and glutathione peroxidase level in experimental lens induced uveitis. Int Ophthalmol 18, 21–24 (1994). https://doi.org/10.1007/BF00919409

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  • DOI: https://doi.org/10.1007/BF00919409

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