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Journal of Clinical Immunology

, Volume 13, Issue 2, pp 152–161 | Cite as

Dual expression of CD45RA and CD45RO isoforms on myelin basic protein-specific CD4+ T-cell lines in multiple sclerosis

  • Yufen Qin
  • Stanley van den Noort
  • Jeff Kurt
  • Sudhir Gupta
Original Articles

Abstract

Myelin basic protein (MBP)-specific T-cell lines from patients with multiple sclerosis (MS) and healthy controls were analyzed for the expression of CD45 isoforms and adhesion molecules. In the multiple sclerosis group, 22 of 24 MBP-specific T-cell lines were CD4+. Two distinct patterns were observed with regard to CD45 isoform expression. Pattern I showed dual expression of CD45 isoforms (CD4+CD45RA+CD45RO+CD29+) and Pattern II included cells with a single CD45 isoform (CD4+CD45RA−CD45RO+CD29+). All 10 cell lines from healthy controls were CD4+ and displayed Pattern II (CD4+CD45RA−CD45RO+CD29+). The dual expression of CD45 isoform in T-cell lines from MS was stable, did not represent a transition stage from CD45RA to CD45RO, and was cell-cycle independent. All cell lines from MS and controls expressed increased levels of LFA-1 (CD11a), LFA-2 (CD2), LFA-3 (CD58), ICAM-1 (CD54), and VLA-4 (CDw49d). These data show the presence of unique MBP-specific T cells (CD4+CD45RA+CD45RO+CD29+) that might play a role in the pathogenesis of MS.

Key words

Multiple sclerosis CD4+ T-cell subsets naive and memory T cells adhesion molecules 

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Copyright information

© Plenum Publishing Corporation 1993

Authors and Affiliations

  • Yufen Qin
    • 1
  • Stanley van den Noort
    • 2
  • Jeff Kurt
    • 1
  • Sudhir Gupta
    • 1
  1. 1.Division of Basic and Clinical Immunology, Department of MedicineUniversity of CaliforniaIrvine
  2. 2.Department of NeurologyUniversity of CaliforniaIrvine

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