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An immunohistochemical study of opsin in photoreceptor cells following light-induced retinal degeneration in the rat

  • Deepak P. Edward
  • Katherine Lim
  • Shoichi Sawaguchi
  • Mark O. M. Tso
Laboratory Investigations

Abstract

Light-induced retinal degeneration has been hypothesized to be rhodopsin-mediated. However, the alterations induced in the opsin moiety of the rhodopsin molecule and its distribution in the rod cell after a photic insult have not been definitively established. We used light and electron immunohistochemistry to study the alterations in retinal opsin immunoreactivity in a rat model of retinal photic injury. In normal unexposed rat retinas, opsin immunoreactivity was restricted to the rod outer segments. At 6 h after a 24-h light exposure, opsin immunoreactivity was present in the rod outer segments in both the superior and inferior retina, but in addition marked immunoreactivity was present in the inner segments in the superior quadrant of the light-damaged retina. At 6 days after exposure, intense immunoreactivity was noted around the severely degenerating rod nuclei and inner segments. However, at 21 days following light exposure, opsin immunoreactivity in areas of recovery was again restricted to the short regenerated rod outer segments. It appears that, despite severe light-mediated retinal degeneration, anti-opsin immunoreactivity persisted in the photoreceptor cells but with an altered pattern in damaged rod outer segments and photoreceptor perikarya. However, opsin immunoreactivity relocated to the regenerated rod outer segments in the recovery phase.

Keywords

Retina Light Exposure Photoreceptor Cell Retinal Degeneration Intense Immunoreactivity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Bowes C, Van Veen T, Farber DB (1988) Opsin, G-protein and 48-kDa protein in normal and rd mouse retinas: developmental expression of mRNAS and proteins and light/dark cycling of mRNAs. Exp Eye Res 47:369–390Google Scholar
  2. 2.
    Erickson PA, Anderson DH, Fisher SK (1987) Use of uranyl acetate en bloc to improve tissue preservation and labeling for post-embedding immunoelectron microscopy. J Electron Microsc Tech 5:303:314Google Scholar
  3. 3.
    Hagins WA, Penn RD, Yoshikami S (1970) Dark current and photocurrent in retinal rods. Biophys J 10:380–412Google Scholar
  4. 4.
    Hargrave PA, Adamus G, Arendt A, McDowell JH, Wang J, Szary A, Curtis D, Jackson RW (1986) Rhodopsin's amino terminus is a principal antigenic site. Exp Eye Res 42:363–373Google Scholar
  5. 5.
    Hicks D, Barnstable CJ (1987) Different rhodopsin monoclonal antibodies reveal different binding patterns on developing and adult rat retina. J Histochem Cytochem 35:1317–1328Google Scholar
  6. 6.
    Hicks D, Sparrow J, Barnstable CJ (1989) Immunoelectron microscopical examination of the surface distribution of opsin in rat rod photoreceptor cells. Exp Eye Res 49:13–29Google Scholar
  7. 7.
    Hsu SM, Raine L, Fanger H (1981) The use of avidin-biotinperoxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580Google Scholar
  8. 8.
    Hubbard R, Bownds D, Yoshizawa T (1965) The chemistry of visual photoreception. Cold Spring Harb Symp Quant Biol 30:301–315Google Scholar
  9. 9.
    Li ZY, Tso MOM, Wang HM, Organisciak DT (1985) Amelioration of photic injury in rat retina by ascorbic acid: a histophatologic study. Invest Ophthalmol Vis Sci 26:1589–1598Google Scholar
  10. 10.
    Maddox PH, Jenkins D (1987) 3-Aminopropyl triethoxysilane (APES): a new advance in section adhesion. J Clin Pathol 40:1256–1257Google Scholar
  11. 11.
    Molday RS (1988) Monoclonal antibodies to rhodopsin and other proteins of rod outer segments. In: Osborne N, Chader G (eds) Progress in retinal research, vol 8. Pergamon, New York, pp 173–209Google Scholar
  12. 12.
    Nir I, Papermaster DS (1986) Immunocytochemical localization of opsin in the inner segment and ciliary plasma membrane of photoreceptors in retinas of rds mutant mice. Invest Ophthalmol Vis Sci 27:836–840Google Scholar
  13. 13.
    Nir I, Papermaster DS (1989) Immunocytochemical localization of opsin in degenerating photoreceptors of RCS rats and rd and rds mice. In: La Vail MM, Anderson RE, Hollyfield JG (eds) Inherited and environmentally induced retinal degenerations (Progress in clinical and biological research, vol 314). Liss, New York, pp 251–264Google Scholar
  14. 14.
    Nir I, Sagie G, Papermaster DS (1987) Opsin accumulation in photoreceptor inner segment plasma membranes of dystrophic RCS rats. Invest Ophthalmol Vis Sci 28:62–69Google Scholar
  15. 15.
    Nir I, Agarwal N, Sagie G, Papermaster DS (1989) Opsin distribution and synthesis in degenerating photoreceptors of rd mutant mice. Exp Eye Res 49:403–421Google Scholar
  16. 16.
    Noell WK (1980) Possible mechanisms of photoreceptor damage by light in mammalian eyes. Vision Res 20:1163–1171Google Scholar
  17. 17.
    Noell WK, Walker VS, Kang BS, Berman S (1966) Retinal damage by light in rats. Invest Ophthalmol Vis Sci 5:450–472Google Scholar
  18. 18.
    Organisciak DT, Xie A, Reeves DS, Donoso LA (1989) Intenselight mediated changes in rat rod outer segment lipids and proteins. In: LaVail MM, Anderson RE, Hollyfield JG (eds) Inherited and environmentally induced retinal degenerations (Progress in clinical and biological research, vol 314). Liss, New York, pp 493–512Google Scholar
  19. 19.
    Rapp LM, Naash ML, Weigand RD, Joel CD, Nielsen JC, Anderson RE (1985) Morphological and biochemical comparisons between retinal regions having differing susceptibility to photoreceptor degeneration. In: Lavail MM (ed) Retinal degeneration: experimental and clinical studies. Liss, New York, pp 412–418Google Scholar
  20. 20.
    Sarthy PV, Fu M (1989) Transcriptional activation of an intermediate filament protein gene in mice with retinal dystrophy. DNA 8:437–446Google Scholar
  21. 21.
    Schnapf JL, Baylor DA (1987) How photoreceptor cells respond to light. Sci Am 256:40–47Google Scholar
  22. 22.
    Shallal A, McKechnie NM, Al-Mahdawi S (1988) Immunochemistry of the outer retina. Eye 2:S180–5201Google Scholar
  23. 23.
    Stryer L (1987) The molecules of visual excitation. Sci Am 257:42–50Google Scholar
  24. 24.
    Tso MOM, Woodford BJ (1983) Effect of photic injury on the retinal tissues. Ophthalmology 90:952–963Google Scholar
  25. 25.
    Usukura J, Bok D (1987) Changes in the localization and content of opsin during retinal development in the rds mutant mouse: immunocytochemistry and immunoassay. Exp Eye Res 45:501–515Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Deepak P. Edward
    • 1
    • 2
  • Katherine Lim
    • 1
    • 2
  • Shoichi Sawaguchi
    • 1
    • 2
  • Mark O. M. Tso
    • 1
    • 2
  1. 1.Department of Ophthalmology and Visual SciencesGeorgiana Dvorak Theobald Ophthalmic Pathology LaboratoryChicagoUSA
  2. 2.Lions of Illinois Eye Research InstituteUniversity of Illinois at Chicago College of MedicineChicagoUSA

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