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Proliferative vitreoretinopathy : An examination of the involvement of lymphocytes, adhesion molecules and HLA-DR antigens

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Abstract

This paper addresses the molecular basis of interactions between leucocytes, other cells in the vitreoretinal environment and extracellular matrix that may underlie the pathogenesis of proliferative vitreoretinopathy. In this study we report the expression of adhesion molecules (CD11a, CD11c, CD18 and ICAM-1), lymphocyte surface markers (CD3, CD4, CD8 and CD22) and HLA-DR molecules in 25 epiretinal membranes obtained from eyes undergoing vitrectomy for the treatment of retinal detachment complicated by epiretinal membrane formation. Retinas from normal cadaveric eyes were used as controls. The results showed that cells expressing the adhesion molecules CD11a, CD11c and CD18 were present in 5 of 25, 17 of 25 and 11 of 23 membranes, respectively. Cells stained with antibodies against intracellular adhesion molecule 1 (ICAM-1) were observed in 24 of 25 membranes, whilst HLA-DR positive cells were seen in all membranes investigated. Immunohistochemical staining revealed that the molecules ICAM-1 or HLA-DR were not only expressed on inflammatory cells but also distributed within the extracellular matrix in several specimens. Lymphocytes expressing CD3 markers were present in 12 of 25 membranes, whilst T lymphocytes expressing CD4 and CD8 markers were observed in 5 of 18 and 12 of 24 membranes, respectively. In contrast, B lymphocytes expressing CD22 molecules were not found in any of the membranes. Leucocyte surface molecules were not expressed in control cadaveric retinas, although occasional cells expressing ICAM-1 were identified in the inner plexiform layer. The present findings indicate that selective interaction between infiltrating leucocytes and adhesion molecules present in extracellular matrix may well be involved in epiretinal membrane formation, and that T lymphocytes may play an important role in the pathogenesis of proliferative vitreoretinopathy.

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Author notes

  1. G. A. Limb

    Present address: Immunology Research Unit, The Rayne Institute, St. Thomas Hospital, Lambeth Palace Road, SEI 7EH, London, UK

Authors and Affiliations

  1. Department of Immunology, St. Thomas Hospital, UMDS, SE1 7EH, London, UK

    G. A. Limb, K. R. Munasinghe & D. C. Dumonde

  2. Eye Department, St. Thomas' Hospital, UMDS, SEI 7EH, London, UK

    Franks W. A. A. & A. H. Chignell

Authors
  1. G. A. Limb
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  2. Franks W. A. A.
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  3. K. R. Munasinghe
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  4. A. H. Chignell
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  5. D. C. Dumonde
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Limb, G.A., Franks, W.A.A., Munasinghe, K.R. et al. Proliferative vitreoretinopathy : An examination of the involvement of lymphocytes, adhesion molecules and HLA-DR antigens. Graefe's Arch Clin Exp Ophthalmol 231, 331–336 (1993). https://doi.org/10.1007/BF00919029

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  • DOI: https://doi.org/10.1007/BF00919029

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