Advertisement

Inflammation

, Volume 13, Issue 1, pp 47–58 | Cite as

Fluoride activation of neutrophils

Similarities to formylmethionyl-leucyl-phenylalanine
  • Walter L. Gabler
  • Howard R. Creamer
  • Wesley W. Bullock
Original Articles

Abstract

Fluoride induced degranulation of both primary and specific granules from neutrophils pretreated with cytochalasin B. There was a similarity in the dependency on extracellular Ca2+ for fluoride- and for FMLP-stimulated O2 generation and degranulation. Pertussis toxin, but not cholera toxin, inhibited FMLP and fluoride activation of neutrophils, while neither toxin affected PMA activation of these cells. These results suggest that fluoride and FMLP activate neutrophils through a common Ca2+-dependent and pertussis toxin-sensitive pathway.

Keywords

Public Health Internal Medicine Fluoride Pertussis Cholera 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Goldstein, I. M., S. Hoffstein, J. Gallin, andG. Weissmann. 1973. Mechanisms of lysosomal enzyme release from human leukocytes: Microtubule assembly and membrane fusion.Proc. Natl. Acad. Sci. U.S.A. 70:2916.Google Scholar
  2. 2.
    Cheson, B. D., R. L. Christensen, R. Sperling, B. E. Kohler, andB. M. Babior. 1976. The origin of the chemiluminescence of phagocytosing granulocytes.J. Clin. Invest. 58:789.Google Scholar
  3. 3.
    Curnutte, J. T., B. M. Babior, andM. L. Karnovsky. 1979. Fluoride-mediated activation of the respiratory burst in neutrophils. A reversible process.J. Clin. Invest. 63:637.Google Scholar
  4. 4.
    Elferink, J. G. R., E. J. J. Alsbach, andJ. C. Riemersma. 1980. The interaction of fluoride with rabbit polymorphonuclear leukocytes: Induction of exocytosis and cytolysis.Biochem. Pharmacol. 29:3051.Google Scholar
  5. 5.
    Harvath, L., H. J. Amirault, andB. R. Anderson. 1978. Chemiluminescence of human and canine polymorphonuclear leukocytes in the absence of phagocytosis.J. Clin. Invest. 61:1145.Google Scholar
  6. 6.
    Goldstein, I. M. 1984. Neutrophil degranulation.Contemp. Top. Immunobiol. 14:189.Google Scholar
  7. 7.
    Smith, R. J., B. J. Bowman, andS. S. Iden. 1982. Pepstatin A-induced degranulation and superoxide anion generation by human neutrophils.Clin. Immunol. Immunopathol. 22:83.Google Scholar
  8. 8.
    Bullock, W. W., H. R. Creamer, andW. L. Gabler. 1982. A rapid quantitative assay for activated neutrophils.Inflammation 6:305.Google Scholar
  9. 9.
    Gabler, W. L., H. R. Creamer, andW. W. Bullock. 1986. Role of extracellular calcium in neutrophil responsiveness to chemotactic tripeptides.Inflammation 10:281.Google Scholar
  10. 10.
    Brittinger, G., R. Hirschhorn, S. D. Douglas, andG. Weissmann. 1968. Studies on lysosome. XI. Characterization of a hydrolase-rich fraction from human lymphocytes.J. Cell Biol. 37:394.Google Scholar
  11. 11.
    Becker, E. L., D. M. Henson, H. J. Showell, andL. S. Hsu. 1974. The ability of chemotactic factors to induce lysosomal enzyme release. I. The characteristics of release, the importance of surfaces, and the relation of release to chemotactic responsiveness.J. Immunol. 112:2047.Google Scholar
  12. 12.
    Creamer, H. R., W. L. Gabler, andW. W. Bullock. 1983. The endogenous component chemotactic assay (ECCA).Inflammation 7:321.Google Scholar
  13. 13.
    Gabler, W. L., andP. A. Leong. 1979. Fluoride inhibition of polymorphonuclear leukocytes.J. Dent. Res. 58:1933.Google Scholar
  14. 14.
    Becker, E. L., M. Sigman, andJ. M. Oliver. 1979. Superoxide production induced in rabbit polymorphonuclear leukocytes by synthetic chemotactic peptides and A23187.Am. J. Pathol. 95:81.Google Scholar
  15. 15.
    Becker, E. L., andH. J. Showel. 1974. The ability of chemotactic factors to induce lysosomal enzyme release. II. The mechanism of release.J. Immunol. 112:2055.Google Scholar
  16. 16.
    Sutherland, E. W., T. W. Rall, andT. Menon. 1962. Adenyl cyclase. I. Distribution, preparation, and properties.J. Biol. Chem. 237:1220.Google Scholar
  17. 17.
    Downes, R. W. Jr., A. M. Spiegel, M. Singer, S. Reen, andG. D. Aurback. 1980. Fiuoride stimulation of adenylate cyclase is dependent on the guanine nucleotide regulatory protein.J. Biol. Chem. 255:949.Google Scholar
  18. 18.
    Howlett, A. C., P. C. Sternweiss, B. A. Macik, P. M. Van Arsdale, andA. G. Gilman. 1979. Reconstitution of catecholamine-sensitive adenyl cyclase.J. Biol. Chem. 254:2287.Google Scholar
  19. 19.
    Synderman, R., C. D. Smith, andM. W. Verghese. 1986. Model for leukocyte regulation by chemoattractant receptors: Roles of a guanine nucleotide regulatory protein and polyphosphoinositide metabolism.J. Leuk. Biol. 40:785.Google Scholar
  20. 20.
    Lad, P. M., B. J. Goldberg, P. A. Smiley, andC. V. Olson. 1985. Receptor-specific threshold effects of cyclic AMP are involved in the regulation of enzyme release and superoxide production from human neutrophils.Biochim. Biophys. Acta 846:286.Google Scholar
  21. 21.
    Wong, K. 1983. The interactive effects of fluoride andN-formyl-l-methionyl-l-leucyl-l-phenylalanine on superoxide production and cAMP levels in human neutrophils.Can. J. Biochem. 61:569.Google Scholar
  22. 22.
    Lad, P. M., C. V. Olson, I. S. Grewal, andS. J. Scott. 1985. A pertussis toxin-sensitive GTP-binding protein in the human neutrophil regulates multiple receptors, calcium mobilization, and lectin-induced capping.Proc. Natl. Acad. Sci. U.S.A. 82:8643.Google Scholar
  23. 23.
    Lad, P. M., C. V. Olson, andP. A. Smiley. 1985. Association of the N-formyl-met-leu-phe receptor in human neutrophils with a GTP-binding protein sensitive to pertussin toxin.Proc. Natl. Acad. Sci. U.S.A. 82:869.Google Scholar
  24. 24.
    Niedel, J. E., L. J. Kuhn, andG. R. Vandenbark. 1983. Phorbol diester receptor copurifies with protein kinase C.Proc. Natl. Acad. Sci. U.S.A. 80:36.Google Scholar
  25. 25.
    Korchak, H. M., L. E. Rutherford, andG. Weissmann. 1984. Stimulus response coupling in the human neutrophil. I. Kinetic analysis of changes in calcium permeability.J. Biol. Chem. 259:4070.Google Scholar
  26. 26.
    Drescher, M., andJ. W. Suttie. 1972. Intracellular fluoride in cultured mammalian cells.Proc. Soc. Exp. Biol. Med. 139:228.Google Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • Walter L. Gabler
    • 1
  • Howard R. Creamer
    • 2
  • Wesley W. Bullock
    • 2
  1. 1.Departments of BiochemistryOregon Health Sciences UniversityPortland
  2. 2.Oral Microbiology/ImmunologyOregon Health Sciences UniversityPortland

Personalised recommendations