Abstract
Leukopenia and pulmonary leukostasis are prominent features in patients succumbing to pneumococcal (PNC) infections. We examined mechanisms involved in recruitment of polymorphonuclear neutrophils (PMNs) into pulmonary capillaries and alveolae after PNC sonicate injection. We showed that by 15 min postinjection, PMN chemotactic activity was found in bronchoalveolar lavage (BAL) fluids and increased with time until the end point of the study at 90 min. Accompanying the increased chemotactic activity in BAL fluids was a decrease in circulating PMNs more pronounced in the femoral artery (FA) then the pulmonary artery (PA). Super-oxide anion (O −2 ) production by peripheral PMNs was depressed following PNC sonicate injection, and comparison of FA and PA showed that FA PMNs produced less O −2 than PA PMNs. PA PMNs also showed enhanced random migration when compared to the depressed random migration of FA PMNs. This study demonstrated that an intravascular challenge of PNC sonicate was associated with increased chemotactic activity for PMNs in BAL fluid. Fewer PMNs and altered PMN function resulted from passage through the pulmonary microvasculature after PNC sonicate injection.
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Supported in part by USDHHS grant CA 20819 from the National Cancer Institute and from the Veterans Administration Research Service.
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Jutila, C.K., Jutila, M.A., Crowell, R.E. et al. Neutrophil responses to intravascular pneumococcal sonicate. Inflammation 16, 135–146 (1992). https://doi.org/10.1007/BF00918953
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DOI: https://doi.org/10.1007/BF00918953