Inflammation

, Volume 2, Issue 2, pp 165–177 | Cite as

Effect of leukocyte hydrolases on bacteria

X. The role played by leukocyte factors, cationic polyelectrolytes, and by membrane-damaging agents in the lysis ofStaphylococcus aureus: Relation to chronic inflammatory procesess
  • Meir Lahav
  • Isaac Ginsburg
Article

Abstract

A heat-stable factor present in extracts of human blood leukocytes is capable of lysing youngStaphylococcus aureus at pH 5.O. Lysis is characterized by breakdown of cell-wall components as judged by electron microscopic and biochemical analysis. The leukocyte extracts can be replaced by a variety of agents known to injure cell membranes, e.g., leukocyte cationic protein histone, polymyxin B, colimycin, phospholipase A, and lysolecithin. The mechanisms by which all these agents bring about the degradation of the staphylococcal walls was studied. By using14C-labeled cell walls devoid of cytoplasmic structures, it was demonstrated that none of the above-mentioned agents had a direct lytic effect on purified cell walls. On the other hand, when any of these agents first interacted with intact staphylococci, a factor (presumably an autolysin) was generated that directly lysed the cell walls. Lysis of cell walls in the presence of intact staphylococci used as a source of autolysin was strongly inhibited by a variety of anionic polyelectrolytes such as heparine and liquoid. The possible role played by bacterial autolysins in the generation of microbial cell-wall components capable of triggering chronic inflammation is discussed.

Keywords

Cell Wall Chronic Inflammation Biochemical Analysis Human Blood Polymyxin 

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References

  1. 1.
    Archibald, A.R. 1972. The chemistry of staphylococcal cell walls.In The staphylococci. J.O. Cohen, editor. Interscience, New York. 75–109.Google Scholar
  2. 2.
    Beckerdite, S., C. Mooney, J. Weiss, R. Franson, andP. Elsbach. 1977. Early and discrete changes in permeability ofEscherichia coli and certain other Gram-negative bacteria during killing by granulocytes.J. Exp. Med. 140:396–409.Google Scholar
  3. 3.
    Efrati, C., T. Sacks, N. Ne'eman, M. Lahav, andI. Ginsburg. 1976. The effect of leukocyte hydrolases on bacteria. VIII. The combined effect of leukocyte extracts, lysozyme, enzyme “cocktails,” and penicillin on the lysis ofStaphylococcus aureus and group A streptococci in vitro.Inflammation 1:371–407.Google Scholar
  4. 4.
    Ehrlich, J., M.Lahav, and M.N.Sela. 1976. The bacteriolytic effect of human dentoalveolar purulent exudates and leukocyte extracts. Submitted for publication.Google Scholar
  5. 5.
    Franson, S.A., P. Patriarca, andP. Elsbach. 1974. Phospholipid metabolism by phagocytic cells. Phospholipases A2 associated with rabbit polymorphonuclear leukocyte granules.J. Lipid Res. 15:380–388.Google Scholar
  6. 6.
    Ginsburg, I., N. Ne'eman, Z. Duchan, M.N. Sela, J.M. James, andM. Lahav. 1975. The effect of leukocyte hydrolases on bacteria. IV. The role played by artificial enzyme “cocktails” and tissue enzymes in bacteriolysis.Inflammation 1:45–56.Google Scholar
  7. 7.
    Ginsburg, I., M.Lahav, N.Ne'eman, Z.Duchan, S.Chanes, and M.N.Sela. The interaction of leukocytes and their hydrolases with bacteria in vitro and in vivo: The modification of the bactericidal and bacteriolytic reactions by cationic and anionic macromolecular substances and by anti-inflammatory agents.Agents Actions 6:292–305.Google Scholar
  8. 8.
    Ginsburg, I., M. Lahav, N. Ne'eman, andJ.M. James. 1974. The inhibition by basic and acidic polyelectrolytes of the degradation of bacteria by leukocyte enzymes: Relation to the persistence of microbial constituents in inflammatory sites.In Activation of Macrophages. W.H. Wagner, H. Hahn, and R. Evans, editors. Excerpta Medica, Amsterdam. 162–169.Google Scholar
  9. 9.
    Ginsburg, I., andM.N. Sela. 1976. The role of leukocytes and their hydrolases in the persistence, degradation and transport of bacterial constituents in tissues: Relation to chronic inflammatory processes in staphylococcal, streptococcal and mycobacterial infections and in chronic periodontal disease.Crit. Rev. Microbiol. 4:249–331.Google Scholar
  10. 10.
    Koga, T., S. Rotani, T. Narita, andC.M. Pearson. 1976. Induction of adjuvant arthritis in the rat by various bacterial cell walls and their water-soluble components.Int. Arch. Allergy Appl. Immunol. 51:206.Google Scholar
  11. 11.
    Lahav, M., N. Ne'eman, E. Adler, andI. Ginsburg. 1974. Effect of leukocyte hydrolases on bacteria. I. Degradation of14C-labeIed streptococcus and staphylococcus.J. Infect. Dis. 129:528–537.Google Scholar
  12. 12.
    Lahav, M., N. Ne'eman, J. James, andI. Ginsburg. 1975. The effect of leukocyte hydrolases on bacteria. III. Bacteriolysis induced by extracts of different leukocyte populations and the inhibition of lysis by macromolecular substances.J. Infect. Dis. 131:149–157.Google Scholar
  13. 13.
    Ne'eman, N., M. Lahav, andI. Ginsburg. 1974. The effect of leukocyte hydrolases on bacteria. II. The synergistic action of lysozyme and extracts of PMN, macrophages, lymphocytes and platelets in bacteriolysis.Proc. Soc. Exp. Biol. Med. 146:1137–1145.Google Scholar
  14. 14.
    Ne'eman, N., Z. Duchan, M. Lahav, M.N. Sela, andI. Ginsburg. 1976. The effect of leukocyte hydrolases on bacteria. VII. Bactericidal and bacteriolytic reactions mediated by leukocyte and tissue extracts and their modifications by polyelectrolytes.Inflammation 1:261–284.Google Scholar
  15. 15.
    Ne'eman, N., andI. Ginsburg. 1972. Red cell sensitizing antigen of group A streptococci. II. Immunological and immunopathological properties.Isr. J. Med. Sci. 8:1807–1816.Google Scholar
  16. 16.
    Page, R.C., P. Davies, andA.C. Allison. 1974. Participation of mononuclear phagocytes in chronic inflammatory diseases.J. Reticuloendothel. Soc. 15:413–438.Google Scholar
  17. 17.
    Salton, M.R.J., andJ.G. Pavlik. 1960. Studies of the bacterial cell wall. VI. Wall composition and sensitivity to lysozyme.Biochim. Biophys. Acta 39:398–407.Google Scholar
  18. 18.
    Schwab, J.H., W.J. Cromartie, S.H. Ohanian, andJ.E. Craddock. 1967. Association of experimental chronic arthritis with the persistence of group A streptococcal cell walls in the articular tissue.J. Bacteriol. 94:1728–1735.Google Scholar
  19. 19.
    Sela, M.N., M. Lahav, N. Ne'eman, Z. Duchan, andI. Ginsburg. 1975. The effect of leukocyte hydrolases on bacteria. V. Modification of bacteriolysis by antiinflammatory agents and by cationic and anionic polyelectrolytes.Inflammation 1:57–69.Google Scholar
  20. 20.
    Sela, M.N., M. Lahav, andI. Ginsburg. 1977. The effect of leukocyte hydrolases on bacteria. IX. The release of lipoteichoic acid from group A streptococci and fromStrep, mutans by leukocyte extracts and by lysozyme.Inflammation 2:151–164.Google Scholar
  21. 21.
    Stein, H., R. Yarom, S. Levine, T. Dishon, andI. Ginsburg. 1973. Chronic self-perpetuating arthritis induced in rabbits by a cell free extract of group A streptococci.Proc. Soc. Exp. Biol. Med. 143:1106–1112.Google Scholar
  22. 22.
    Strominger, J.L., andGhusen, J.M. 1967. Mechanisms of enzymatic bacteriolysis.Science 156:213–217.Google Scholar
  23. 23.
    Suganuma, A., 1972. Fine structure of staphylococci: Electron microscopy.In The Staphylococci. J.O. Cohen, editor. Interscience, New York. 213–217.Google Scholar
  24. 24.
    Takeba, I., H.J. Singer, E.M. Wise, andJ.T. Park. 1970.Staphylococcus aureus H. autolytic activity: General properties.J. Bacteriol. 102:14–19.Google Scholar
  25. 25.
    Tipper, D.J. 1969. Mechanism of autolysis of isolated cell walls ofStaphylococcus aureus.J. Bacteriol. 97:837.Google Scholar
  26. 26.
    Wadström, T., andK. Hisatsune. 1970. Bacteriolytic enzymes fromStaphylococcus aureus. Specificity of action of endo-β-N-acetylglucosaminidase.Biochem. J. 120:735–744.Google Scholar
  27. 27.
    Wadström, T. 1973. Bacteriolytic enzymes from staphylococci. Contributions to Microbiology and Immunology. Vol. 1. Staphylococci and Staphylococcai Infections. Karger, Basel. 397–405.Google Scholar
  28. 28.
    Weiss, J., R.C. Franson, S. Beckerdite, K. Schmeidler, andP. Elsbach. 1975. Partial characterization and purification of a rabbit granulocyte factor that increases permeability ofEscherichia coll.J. Clin. Invest. 55:33–42.Google Scholar
  29. 29.
    Weiss, J., R.C. Franson, J. Schmeidler, andP. Elsbach. 1976. Reversible envelope effects during and after killing ofEscherichia coli W by a highly purified rabbit polymorphonuclear leukocyte fraction.Biochim. Biophys. Acta 436:154–169.Google Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • Meir Lahav
    • 1
  • Isaac Ginsburg
    • 1
  1. 1.Department of Oral BiologyHebrew University-Hadassah School of Dental Medicine, Founded by the Alpha Omega FraternityJerusalemIsrael

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