, Volume 2, Issue 2, pp 105–113 | Cite as

The purification and characterization of a lymphokine chemotactic for lymphocytes-lymphotactin

  • John C. Houck
  • Catherine M. Chang


Aqueous extracts of the thymus from vaccinated calves contain a protein specifically chemotactic for lymphocytes (lymphotactin). Lymphotactin has been isolated and purified by ethanol fractionation, ultrafiltration, isoelectric focusing, and preparative acrylamide gel electrophoresis. Its isoelectric point is 5.9, and the molecular weight by both SDS electrophoresis and exclusion chromatography is about 10,500. The purified sialoprotein is homogeneous at three different pH's in acrylamide gel electrophoresis. As little as 1μg of this material intraperitoneally will produce massive lymphocyte infiltration within 4 h in vivo.


Public Health Molecular Weight Internal Medicine Acrylamide Aqueous Extract 
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Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • John C. Houck
    • 1
  • Catherine M. Chang
    • 1
  1. 1.Virginia Mason Research CenterSeattle

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