TCA manual / Tissue Culture Association

, Volume 3, Issue 2, pp 601–605 | Cite as

Two procedures for estimating mean cell volume

  • Peter C. Will
  • Emily T. Brake
  • John S. Cook


Cell Volume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Simons, J. W. I. M. 1967. The use of frequency distributions of cell diameters to characterize cell populations in tissue culture. Exp. Cell Res. 45: 336–350.Google Scholar
  2. 2.
    Anderson, E. C., and D. F. Petersen. 1967. Cell growth and division. II. Experimental studies of cell volume distributions in mammalian suspension cultures. Biophys. J. 7: 353–364.Google Scholar
  3. 3.
    Bell, G. I., and E. C. Anderson. 1967. Cell growth and division. I. A mathematical model with applications to cell volume distributions in mammalian suspension cultures. Biophys. J. 7: 329–351.Google Scholar
  4. 4.
    Epstein, S. S., and J. B. Weiss. 1960.Measuring the size of isolated cells. Nature 187: 461–463.Google Scholar
  5. 5.
    Peacock, A. C., G. Z. Williams, and H. F. Mengoli. 1960. Rapid electronic measurement of cell volume and distribution. J. Natl. Cancer Inst. 25: 63–74.Google Scholar
  6. 6.
    Simons, J. W. I. M. 1970. Characterization of somatic cells by determination of their volumes with a Coulter counter. J. Cell Biol. 46: 610–612.Google Scholar
  7. 7.
    Brecher, G., M. Schneiderman, and E. P. Cronkite. 1953. The reproducibility and constancy of the platelet count. Am. J. Clin. Pathol. 23: 15–26.Google Scholar
  8. 8.
    Tolnai, S. 1975. A method for viable cell count. Procedure 70111. TCA Manual 1: 37–38.Google Scholar
  9. 9.
    Merchant, D. J., R. H. Kahn, and W. H. Murphy. 1964.Handbook of Cell and Organ Culture. 2nd ed. Burgess Publishing Co., Minneapolis.Google Scholar
  10. 10.
    Jacobs, M. H., and A. K. Parpart. 1931. Osmotic properties of the erythrocyte. II. The influence of pH, temperature, and oxygen tension on hemolysis by hypotonic solutions. Biol. Bull. 60: 95–119.Google Scholar
  11. 11.
    Wattiaux, R., S. Wattiaux-De Coninck, M-J. Rutgeerts, and P. Tulkens. 1964. Influence of the injection of a sucrose solution on the properties of rat-liver lysosomes. Nature 203: 757–758.Google Scholar
  12. 12.
    Cohn, Z. A., and B. A. Ehrenreich. 1969. The uptake, storage, and intracellular hydrolysis of carbohydrates by macrophages. J. Exp. Med. 129: 201–225.Google Scholar
  13. 13.
    Bernacki, R. J., and H. B. Bosmann. 1971. The effects of sucrose and various salts on the growth and lysosomal enzyme activity of L5178Y cells. J. Cell Sci. 8: 399–406.Google Scholar
  14. 14.
    Steinman, R. M., J. M. Silver, and Z. A. Cohn. 1974. Pinocytosis in fibroblasts. Quantitative studies in vitro. J. Cell Biol. 63: 949–969.Google Scholar
  15. 15.
    Berkson, J., T. B. Magath, and M. Hurn. 1939–1940. The error of estimate of the blood cell count as made with the hemocytometer. Am. J. Physiol. 128: 309–323.Google Scholar
  16. 16.
    Diamond, P. S., and R. F. Denman. 1966.Laboratory Techniques in Chemistry and Biochemistry. Butterworth & Co. Publishers Ltd., London, p. 176.Google Scholar
  17. 17.
    Kennedy, J. A., and G. A. Millikan. 1938. A micro blood volume method using a blue dye and photocell. J. Physiol. 93: 276–284.Google Scholar
  18. 18.
    Stewart, G. N. 1899. The relative volume or weight of corpuscles and plasma in blood. J. Physiol. 24: 356–373.Google Scholar
  19. 19.
    Maizels, M., M. Remington, and R. Truscoe. 1958. Data for the calculation of the rate coefficients of sodium transfer by mouse ascites tumour cells. J. Physiol. 140: 48–60.Google Scholar
  20. 20.
    Bourke, R. S., E. S. Greenberg, and D. B. Tower. 1965. Variation of cerebral cortex fluid spaces in vivo as a function of species brain size. Am. J. Physiol. 208: 682–692.Google Scholar
  21. 21.
    Law, R. O., and C. F. Phelps. 1966. The size of the sucrose, raffinose, and inulin spaces in the gastrocnemius muscle of the rat. J. Physiol. 186: 547–557.Google Scholar
  22. 22.
    Cohen, S. R. 1969. A rapid, sensitive, semimicro gel filtration procedure for detecting and removing low molecular weight fragments from [3H]- or [14C]-labeled inulin. Anal. Biochem. 31: 539–544.Google Scholar
  23. 23.
    McEvoy, A. F., and W. G. Harris 1971. Evaluation of liquid scintillation systems for the assay of tritiated inulin. Anal. Biochem. 43: 123–128.Google Scholar
  24. 24.
    Parker, R. C. 1961.Methods of Tissue Culture. 3rd ed. P. B. Hoeber, Inc., New York, p. 271.Google Scholar

Copyright information

© Tissue Culture Association 1977

Authors and Affiliations

  • Peter C. Will
    • 1
    • 2
  • Emily T. Brake
    • 1
    • 2
  • John S. Cook
    • 1
    • 2
  1. 1.The University of Tennessee-Oak Ridge Graduate School of Biomedical SciencesOak Ridge
  2. 2.Biology DivisionOak Ridge National LaboratoryOak Ridge

Personalised recommendations