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Inflammation

, Volume 8, Issue 4, pp 417–427 | Cite as

Effect of amoxycillin and doxycycline on function of human granulocytes tested in vitro and on chemotaxis of granulocytes from rabbits given the two antibiotics

  • Vincent Grec
  • Philippe C. Frei
Original Articles

Abstract

The effect of amoxycillin and doxycyciine on human granulocyte function was studied in vitro. The antimicrobial agents were added to tests of chemotaxis, random motility, yeast phagocytosis, and killing, at progressing concentrations. The effect was also evaluated in vivo by measuring chemotaxis and random motiiity (agarose technique) of granulocytes from rabbits injected with these antibiotics. Amoxycillin was shown to have a slightly stimulating effect on chemotaxis, demonstrable only at the highest concentration tested (100 μg/ml), and no effect on the other variables, Doxycyciine had a dose-related inhibitory effect on chemotaxis, random motiiity, and phagocytosis, and no effect on killing. Chemotaxis and random motiiity were slightly, but not significantly, stimulated in vivo when rabbits were given amoxycillin. Chemotaxis (but not random motiiity) was significantly impaired when the rabbits were given doxycyciine.

Keywords

Public Health Internal Medicine Agarose Antimicrobial Agent Doxycycline 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Gallin, J. I., andP. G. Quie, editors. 1978. Leucocyte Chemotaxis. Raven Press, New York.Google Scholar
  2. 2.
    Kokubun, Y., editor. 1979. Phagocytosis: Its Physiology and Pathology, University Park Press, Baltimore.Google Scholar
  3. 3.
    Zakhireh, B., L. H. Block, andR. K. Root. 1979. Neutrophil function and host resistance.Infection 7:88–98.Google Scholar
  4. 4.
    Belsheim, J., H. Gnarpe, andS. Persson. 1979. Tetracyclines and host defense mechanisms: Interference with leukocytes chemotaxis.Scand. J. Infect. Dis. 11:141–145.Google Scholar
  5. 5.
    Esterly, N. B., N. L. Furey, andL. E. Flanagan. 1978. The effect of antimicrobial agents on leukocyte chemotaxis.J. Invest. Dermatol. 70:51–55.Google Scholar
  6. 6.
    Forsgren, A., andD. Schmeling. 1977. Effect of antibiotics on chemotaxis of human leukocytes.Antimicrob. Agents Chemother. 11:580–584.Google Scholar
  7. 7.
    Gnarpe, H., J, Belsheim, andS. Persson. 1978. Tetracycline interference with leukocyte chemotaxis in vitro and in vivo.Infection (Suppl 6)1:98–101.Google Scholar
  8. 8.
    Majeski, J. A., M. A. McClellan, andJ. A. Alexander. 1976. Effect of antibiotics on the in vitro neutrophil chemotactic response.Am. Surg. 42:785–788.Google Scholar
  9. 9.
    Majeski, J. A., andJ. W. Alexander. 1977. Evaluation of tetracycline in the neutrophil chemotactic response.J. Lab. Clin. Med. 90:259–265.Google Scholar
  10. 10.
    Plewig, G., andE. Schöpf. 1975. Antiinflammatory effects of antimicrobial agents: An in vivo study.J. Invest. Dermatol. 65:532–536.Google Scholar
  11. 11.
    Gange, R. W. 1980. Neutrophil chemotaxis in the presence of antibiotics: A reevaluation using an agarose technique.Br. J. Dermatol. 103:51–59.Google Scholar
  12. 12.
    Martin, R. R., G. A. Warr, R. B. Couch, H. Yeager, andV. Knight. 1974. Effects of tetracycline on leukotaxis.J. Infect. Dis. 129:110.Google Scholar
  13. 13.
    Forsgren, A., D. Schmeling, andP. G. Quie. 1974. Effect of tetracycline on the phagocytic function of human leukocytes.J. Infect. Dis. 130:412–415.Google Scholar
  14. 14.
    Gnarpe, H., andD. Leslie. 1974, Tetracyclines and host defense mechanisms Doxycycline interference with phagocytosis ofEscherichia coli.Microbiology 10A:127–138.Google Scholar
  15. 15.
    Melby, K., andT. Midtvedt. 1977. The effect of eight antibacterial agents on the phagocytosis of32P-labelledEscherichia coli by rat polymorphonuclear cells.Scand. J. Infect. Dis. 9:9–12.Google Scholar
  16. 16.
    Welch, W. D., D. Davis, andL. D. Thrupp. 1981. Effect of antimicrobial agents on human polymorphonuclear leukocyte microbicidal function.Antimicrob. Agents Chemother. 20:15–20.Google Scholar
  17. 17.
    Seneca, H., andP. Peer. 1966. Effect of antibacterials, antibiotics, enzymes and steroids on phagocytosis.J. Am. Geriatr. Soc. 14:187–199.Google Scholar
  18. 18.
    Hoeprich, P. D., andC. H. Martin. 1970. Effect of tetracycline, polymyxin B, and rifampin on phagocytosis.Clin. Pharmacol. 11:418–422.Google Scholar
  19. 19.
    Butler, T., M. Aikawa, A. Habte-Michael, andC. Wallace. 1980. Phagocytosis ofBorrelia recurrentis by blood polymorphonuclear leukocytes is enhanced by antibiotic treatment.Infect. Immun. 28:1009–1013.Google Scholar
  20. 20.
    Alexander, J. W., andR. A. Good. 1968. Effect of antibiotics on the bacterial activity of human leukocytes.J. Lab. Clin. Med. 71:971–983.Google Scholar
  21. 21.
    Kernbaum, S. 1974. Pouvoir candidacide des polynucléaires neutrophiles humains et chimiothérapie antibactérienne.Pathol. Biol. 22:789–794.Google Scholar
  22. 22.
    Aubertin, J., P. Legendre, B, Portal, J. Y. Lacut, M. Merlet, A. M. Issanchou, andB. Leng. 1977. Antibiotiques et polynucléaires.Med. Mal. Infect. 7:139–144.Google Scholar
  23. 23.
    Macheret, J. P. 1980. Influence de l'amoxycilline, du cotrimoxazole et de la doxycycline ajoutés in vitro sur la réduction du NBT par des granulocytes humains normaux en présence de bactéries. Thesis, Berne.Google Scholar
  24. 24.
    Thomke, E.T., andH. Bürgi. 1978. Beeinflussung der phagozytären Nitroblau-Tetrazolium-Reduktion durch Sputum chronischer Bronchitiker im Verlaufe einer antiinfektiven Therapie.Pharm. Acta Helv. 53:24–32.Google Scholar
  25. 25.
    De Simone, C. M. Manganaro, D. Meli, D. Ricca, andC. Capozzi. 1980. Influenza degli antibiotici sulla migrazione leucocitaria.Boll Ist. Sieroter. Milan. 59:612–618.Google Scholar
  26. 26.
    Nishida, M., Y. Mine, andY. Yokota. 1976. Effect of antibiotics on the phagocytosis and killing ofPseudomonas aeruginosa by rabbit polymorphonuclear leukocytes.Chemotherapy 22:203–209.Google Scholar
  27. 27.
    Rubinstein, A., B. Pelet, L. Schneider, andG. Pestoni. 1973. Der Einfluss antimikrobieller Medikamente auf die Nitroblau-Tetrazolium-(NBT) Reduktion.Schweiz. Rund. Med. Praxis 62:448–450.Google Scholar
  28. 28.
    Nishida, M., Y. Mine, andS. Nonoyama. 1978. Relationship between the effect of carbenicillin on the phagocytosis and killing ofProteus mirabilis by polymorphonuclear leukocytes and therapeutic efficiency.J. Antibiot. 31:719–724.Google Scholar
  29. 29.
    Nishida, M., Y. Mine, S. Nonoyama andH. Koja. 1977. Nocardicin A, a new monocyclic beta-lactam antibiotic. III. In vitro evaluation.J. Antibiot. 30:921–925.Google Scholar
  30. 30.
    Petit, J. C., andG. L. Daguet. 1981. Enhanced killing ofPseudomonas aeruginosa by human polymorphonuclear leukocytes in presence of subinhibitory concentrations of carbenicillin and ticarcillin.Biomedicine 34:29–33.Google Scholar
  31. 31.
    Lehrer, R.I. 1971. Inhibition by sulfonamides of candidacidal activity of human neutrophils.J. Clin. Invest. 50:2498–2505.Google Scholar
  32. 32.
    Kaplan, S.S., P.A. Perrilie, andS. C. Finch. 1969. The effect of chloramphenicol on human leukocyte phagocytosis and respiration,Proc. Soc. Exp. Biol. 130:839–843.Google Scholar
  33. 33.
    Kaplan, S.S., andS.C. Finch. 1970. Studies on the mechanism of chloramphenicol impairment of human leukocyte function.Proc. Soc. Exp. Biol. 134:287–290.Google Scholar
  34. 34.
    Lehrer, R. I. 1973. Effects of colchicine and chloramphenicol on the oxidative metabolism and phagocytic activity of human neutrophils.J. Infect. Dis. 127:40–48.Google Scholar
  35. 35.
    Goodhart, G. L. 1977. Effect of aminoglycosides on the chemotactic response of human polymorphonuclear leukocytes.Antimicrob. Agents Chemother. 12:540–542.Google Scholar
  36. 36.
    Khan, A.J., H. E. Evans, L. Glass, P. Khan, C. T. Chang, andS. R. Nair. 1979. Abnormal neutrophil chemotaxis and random migration induced by aminoglycoside antibiotics.J. Lab. Clin. Med. 33:295–300.Google Scholar
  37. 37.
    Weinstein, L. 1975. Antimicrobial agents.In The Pharmacological Basis of Therapeutics. L. S. Goodman, and A. Gilman, editors. Macmillan, New York. 1130–1158 and 1183–1200.Google Scholar
  38. 38.
    Frei, P. C., M. H. Baisero, andM. Ochsner. 1974. Chemotaxis of human polymorphonuclears in vitro. II. Technical study.J. Immunol. Methods 5:375–386.Google Scholar
  39. 39.
    Regamey, A. 1973. Chimiotactisme des polynucléaires humains “in vitro.” I. Etude de la méthode chez l'adulte normal.Ann. Immoi. Inst. Past. 124C:493–509.Google Scholar
  40. 40.
    Simpson, D. W., R. Roth, andL. D. Loose. 1979. A rapid, inexpensive and easily quantified assay for phagocytosis and microbicidal activity of macrophages and neutrophils.J. Immunol. Methods 29:221–226.Google Scholar
  41. 41.
    Frei, P. C., A. Hermanovicz, andA. PÉcoud. 1978. Chemotaxis of human polymorphonuclears in vitro. V. Role of the nonsegmented neutrophils and of the experimental conditions in the impairment of chemotaxis observed during bacterial infection.J. Lab. Clin. Med. 92:577–583.Google Scholar
  42. 42.
    Bakker-Woudenberg, I.A. J. M., J. Y. T. de Jong-Hoenderop. andM. R. Michel. 1979. Efficacy of antimicrobial therapy in experimental rat pneumonia: Effects of impaired phagocytosis.Infect. Immun. 25:366–374.Google Scholar

Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • Vincent Grec
    • 1
  • Philippe C. Frei
    • 1
  1. 1.Division of Immunology and Allergy, Department of MedicineCentre Hospitalier Universitaire VaudoisLausanneSwitzerland

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